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Dispersion And Fixation Of Adeno-Associated Virus With Glutaraldehyde For Afm Studies

Author

Listed:
  • PENG WANG

    (Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, P. R. China;
    Graduate School of the Chinese Academy of Sciences, Beijing 100049, P. R. China)

  • XINYAN WANG

    (Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, P. R. China;
    Graduate School of the Chinese Academy of Sciences, Beijing 100049, P. R. China)

  • HAIJUN YANG

    (Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, P. R. China;
    Graduate School of the Chinese Academy of Sciences, Beijing 100049, P. R. China)

  • JUNHONG LÜ

    (Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, P. R. China)

Abstract

Sample preparation is an important procedure for atomic force microscope (AFM) studies. However, flexible virus particles have a tendency to aggregate together and are easily compressed during sample preparation or by AFM tip that subsequently hamper studying of virus by AFM. Herein, low concentration chemical reagent of glutaraldehyde (2%,v/v) is pre-mixed in virus suspension that facilitates the dispersion and observation of recombinant serotype 2 adeno-associated virus particles deposited on mica surface with little deformation.

Suggested Citation

  • Peng Wang & Xinyan Wang & Haijun Yang & Junhong Lü, 2008. "Dispersion And Fixation Of Adeno-Associated Virus With Glutaraldehyde For Afm Studies," Surface Review and Letters (SRL), World Scientific Publishing Co. Pte. Ltd., vol. 15(05), pages 595-598.
    • Handle: RePEc:wsi:srlxxx:v:15:y:2008:i:05:n:s0218625x08011688
    DOI: 10.1142/S0218625X08011688
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