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Cellulose degrading capabilities of cellulolytic bacteria isolated from the intestinal fluids of the silver cricket

Author

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  • N. Chakraborty

    (University of Kalyani, Kalyani, Nadia)

  • G. M. Sarkar

    (University of Kalyani, Kalyani, Nadia)

  • S. C. Lahiri

    (University of Kalyani, Kalyani, Nadia)

Abstract

Cellulose degrading bacteria were isolated from the intestinal fluid of the silver cricket Lepisma Sp. and a culture was developed anoxigenically in the cellulose degrading medium following standard laboratory techniques. The cellulolytic activity of the microbe was examined in a broth culture using Whatman 42 filter paper as the source of insoluble cellulose. Activity was measured spectrophotometrically (620nm) following Anthrone reaction of the culture filtrate and the sugar produced was quantified as a factor of time. The pH optimum was found to be between 7 and 8. Temperature profile optimum was between 30–37°C. The microbes appeared as white colonies on a solid medium. Morphologically the bacterium is a gram-positive nonspore forming rod which was tentatively identified as a new strain of Cellulomonas sp.

Suggested Citation

  • N. Chakraborty & G. M. Sarkar & S. C. Lahiri, 2000. "Cellulose degrading capabilities of cellulolytic bacteria isolated from the intestinal fluids of the silver cricket," Environment Systems and Decisions, Springer, vol. 20(1), pages 9-11, March.
    • Handle: RePEc:spr:envsyd:v:20:y:2000:i:1:d:10.1023_a:1006691524607
    DOI: 10.1023/A:1006691524607
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    Cited by:

    1. Nilanjan Chakraborty & G.M. Sarkar & S.C. Lahiri, 2002. "Biomethanation of plant materials and agricultural residues using dung samples as wild population of microbes and also with isolated methanogens," Environment Systems and Decisions, Springer, vol. 22(2), pages 173-182, June.
    2. Fagbohoun Jean Bedel & Yapi Jocelyn Constant & Deffan Zranseu Bénédicte, 2020. "Kinetics and Thermodynamics of Thermal Inactivation of Cellulase from Salivary Glands of Macrotermes subhyalinus Little Soldier," International Journal of Sciences, Office ijSciences, vol. 9(04), pages 13-20, April.

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