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Analysis of morphological, morphokinetics, cell-free DNA, microRNAs parameters to predict aneuploidy status of embryos

Author

Listed:
  • Achmad Kemal Harzif
  • Intan Indah Permatasari
  • Patricia Alika Kurniawan
  • Thalia Amila Elsiyana
  • Fistyanisa Elya Charilda
  • Azizah Fitriayu Andyra
  • Heidi Dewi Mutia
  • Nafi’atul Ummah
  • Putri Nurbaeti
  • Amalia Shadrina
  • Pritta Ameilia Iffanolida
  • Kresna Mutia
  • Ririn Rahmala Febri
  • Retno Asti Werdhani
  • Dwi Anita Suryandari
  • Wisnu Jatmiko
  • Arief Boediono
  • Hartanto Bayuaji
  • R Muharam Natadisastra
  • Budi Wiweko

Abstract

Background: In Vitro Fertilization (IVF) is one of the most effective infertility treatments. Preimplantation genetic testing for aneuploidy (PGT-A) is invasive, high-cost, and has the potential risk of embryo damage. Our study explores noninvasive approaches to assess embryo quality through morphology and morphokinetics and analyze miRNA, cell-free DNA (cfDNA) for embryo chromosomal status. Methods: This cross-sectional study followed the STROBE guideline. We analysed the relationship between embryo morphology, morphokinetics, cfDNA, and microRNAs with the aneuploidy status of day-5 embryos at the blastocyst stage. Non-probability consecutive sampling yielded 124 embryos between 28th December 2021 and 31st December 2022 in our center. Model performance was evaluated using the area under the curve (AUC) from receiver operating characteristic (ROC) analysis. Results: Of 124 embryos, 50.8% had aneuploid chromosomes. Analysis of ccfDNA demonstrated a significantly higher rate of aneuploidy from aneuploid embryos compared to the euploid group (p

Suggested Citation

  • Achmad Kemal Harzif & Intan Indah Permatasari & Patricia Alika Kurniawan & Thalia Amila Elsiyana & Fistyanisa Elya Charilda & Azizah Fitriayu Andyra & Heidi Dewi Mutia & Nafi’atul Ummah & Putri Nurbae, 2026. "Analysis of morphological, morphokinetics, cell-free DNA, microRNAs parameters to predict aneuploidy status of embryos," PLOS ONE, Public Library of Science, vol. 21(4), pages 1-13, April.
    • Handle: RePEc:plo:pone00:0344795
    DOI: 10.1371/journal.pone.0344795
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