IDEAS home Printed from https://ideas.repec.org/a/plo/pone00/0332041.html
   My bibliography  Save this article

Mechanisms responsible for the ability of enoxaparin sodium to inhibit inflammatory responses in the immune microenvironment of bone repair: A transcriptomic sequencing study

Author

Listed:
  • Xiaoyu Shen
  • Qiang Yao
  • Lijie Ma

Abstract

Enoxaparin sodium (ES), a low molecular weight heparin derivative, has recently been recognized for its diverse biological activities. In particular, the ability of heparin to modulate inflammation has been utilized to enhance the biocompatibility of bone implant materials. In this study, we utilized poly (methyl methacrylate) (PMMA), a drug loading bone implant material, as a matrix and combined this with enoxaparin sodium (ES) to create enoxaparin sodium PMMA cement (ES-PMMA) to investigate the regulatory effects of ES on inflammatory responses in bone tissue from an animal model. We established a rabbit model of femoral condyle bone defects to investigate the immunoregulatory mechanisms of ES-PMMA. Rabbits were divided into control (n = 5), model (n = 10), PMMA (n = 10) and ES-PMMA (n = 10) groups. The control group underwent sham surgery as a blank control, while the model group was established with a bone defect model in the rabbit femoral condyle. The PMMA group and ES-PMMA group followed the same modeling procedure as the model group. After successful modeling, the PMMA group and ES-PMMA group were implanted with PMMA bone cement columns and ES-PMMA bone cement columns, respectively. Ten days post-surgery, cancellous bone tissue from the defect site was collected from each group, and the control group was sampled at the same location. Tissue samples were collected from each group for transcriptomic sequencing. RNA sequencing (RNA-seq) was performed and differentially expressed mRNAs were identified between the model and controls, between the PMMA and model groups, and between the ES-PMMA and model groups. Key candidate genes associated with ES-PMMA treatment were identified (304 genes), and Gene Set Variation Analysis (GSVA), Gene Ontology (GO), and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed on the differentially expressed genes and key candidate genes in each group (P

Suggested Citation

  • Xiaoyu Shen & Qiang Yao & Lijie Ma, 2025. "Mechanisms responsible for the ability of enoxaparin sodium to inhibit inflammatory responses in the immune microenvironment of bone repair: A transcriptomic sequencing study," PLOS ONE, Public Library of Science, vol. 20(9), pages 1-28, September.
    • Handle: RePEc:plo:pone00:0332041
    DOI: 10.1371/journal.pone.0332041
    as

    Download full text from publisher

    File URL: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0332041
    Download Restriction: no
    File URL: https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0332041&type=printable
    Download Restriction: no
    File URL: https://libkey.io/10.1371/journal.pone.0332041?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:plo:pone00:0332041. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: plosone (email available below). General contact details of provider: https://journals.plos.org/plosone/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.