IDEAS home Printed from https://ideas.repec.org/a/plo/pone00/0328403.html
   My bibliography  Save this article

Genetic diversity, population structure and quality-trait associations in Blumea balsamifera revealed by EST-SSR markers

Author

Listed:
  • Mei Huang
  • Xin Li
  • Zhenxia Chen
  • Dan Wang
  • Kai Wang
  • Xuan Hu
  • Xiaolu Chen
  • Lingliang Guan
  • Hongrui Zhang
  • Yuxin Pang
  • Fulai Yu

Abstract

Blumea balsamifera is a widely used ancient medicinal herb in tropical and subtropical Asia. Studying the genetic diversity of B. balsamifera germplasm resources and developing molecular markers related to the content of active constituents in B. balsamifera are of great significance for breeding varieties with high active ingredient content. In this study, the contents of 6 key active constituents were determined in 51 B. balsamifera accessions. EST-SSR markers were used to study their genetic diversity. At the same time, genetic structure analysis and linkage disequilibrium analysis were performed. On this basis, association analysis between EST-SSR markers and active ingredient content was performed. Quantitative analysis revealed substantial variation in six bioactive compounds, with coefficients of variation (CV) exceeding 50% across all metabolites. This result revealed its suitability for association analysis. Genetic diversity analysis revealed a total of 102 alleles amplified from 22 pairs of primers, with effective alleles accounting for 53.52%. The average polymorphism information content was 0.488, with 9 pairs of primers exhibiting high polymorphism (PIC > 0.5), 11 pairs of primers showing moderate polymorphism (0.25 0.5), validating the suitability of this germplasm set for association mapping. Association analysis between quality traits and EST-SSR markers showed that, in both GLM and MLM models, Bbf137 and Bbf377 were found to be associated with 3,3’,5,7-tetrahydroxy-4’- methoxyflavanone (TMF, P

Suggested Citation

  • Mei Huang & Xin Li & Zhenxia Chen & Dan Wang & Kai Wang & Xuan Hu & Xiaolu Chen & Lingliang Guan & Hongrui Zhang & Yuxin Pang & Fulai Yu, 2025. "Genetic diversity, population structure and quality-trait associations in Blumea balsamifera revealed by EST-SSR markers," PLOS ONE, Public Library of Science, vol. 20(7), pages 1-16, July.
    • Handle: RePEc:plo:pone00:0328403
    DOI: 10.1371/journal.pone.0328403
    as

    Download full text from publisher

    File URL: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0328403
    Download Restriction: no
    File URL: https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0328403&type=printable
    Download Restriction: no
    File URL: https://libkey.io/10.1371/journal.pone.0328403?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:plo:pone00:0328403. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: plosone (email available below). General contact details of provider: https://journals.plos.org/plosone/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.