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A computer vision based optical method for measuring fluid level in cell culture plates

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  • Pierre V Baudin
  • Mircea Teodorescu

Abstract

For a transparent well with a known volume capacity, changes in fluid level result in predictable changes in magnification of an overhead light source. For a given well size and fluid, the relationship between volume and magnification can be calculated if the fluid’s index of refraction is known or in a naive fashion with a calibration procedure. Light source magnification can be measured through a camera and processed using computer vision contour analysis with OpenCV. This principle was applied in the design of a 3D printable sensing device using a raspberry pi zero and a camera.

Suggested Citation

  • Pierre V Baudin & Mircea Teodorescu, 2023. "A computer vision based optical method for measuring fluid level in cell culture plates," PLOS ONE, Public Library of Science, vol. 18(9), pages 1-12, September.
    • Handle: RePEc:plo:pone00:0290951
    DOI: 10.1371/journal.pone.0290951
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    References listed on IDEAS

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    1. Heidi Fleischer & Daniel Baumann & Shalaka Joshi & Xianghua Chu & Thomas Roddelkopf & Michael Klos & Kerstin Thurow, 2018. "Analytical Measurements and Efficient Process Generation Using a Dual–Arm Robot Equipped with Electronic Pipettes," Energies, MDPI, vol. 11(10), pages 1-21, September.
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