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Comparison of new and old BacT/ALERT aerobic bottles for detection of Candida species

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  • Kye Won Choe
  • Yong Kwan Lim
  • Mi-Kyung Lee

Abstract

Purpose: A new version of aerobic blood culture media has been developed for the BacT/ALERT (bioMérieux) blood culture system. We evaluated the time to detection and yeast cell counts in positive blood cultures for each Candida spp. according to changes in media. Methods: Isolates from defibrinated horse blood were inoculated into three types of bottles: the old version of aerobic bottle, new version of aerobic bottle, and anaerobic bottle. All bottles were incubated in the BacT/ALERT Virtuo blood culture system. The time to detection was monitored for each bottle, and yeast cell counts were performed immediately after testing positive, determined via the plate count method. Clinical retrospective data of the candidemia samples before and after aerobic bottle change also were analyzed. Results: The median time to detection was 52.47 hours in the old aerobic bottles versus 19.92 hours in the new aerobic bottles (P

Suggested Citation

  • Kye Won Choe & Yong Kwan Lim & Mi-Kyung Lee, 2023. "Comparison of new and old BacT/ALERT aerobic bottles for detection of Candida species," PLOS ONE, Public Library of Science, vol. 18(11), pages 1-10, November.
    • Handle: RePEc:plo:pone00:0288674
    DOI: 10.1371/journal.pone.0288674
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