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Standardization of in-house anti-IgG and IgA ELISAs for the detection of COVID-19

Author

Listed:
  • Kamonthip Rungrojcharoenkit
  • Rungarun Suthangkornkul
  • Darunee Utennam
  • Darunee Buddhari
  • Soontorn Pinpaiboon
  • Duangrat Mongkolsirichaikul
  • Stefan Fernandez
  • Anthony R Jones
  • Thomas S Cotrone
  • Taweewun Hunsawong

Abstract

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of coronavirus disease 2019 (COVID-19). RT-PCR detection of viral RNA represents the gold standard method for diagnosis of COVID-19. However, multiple diagnostic tests are needed for acute disease diagnosis and assessing immunity during the COVID-19 outbreak. Here, we developed in-house anti-RBD IgG and IgA enzyme-linked immunosorbent assays (ELISAs) using a well-defined serum sample panel for screening and identification of human SARS-CoV-2 infection. We found that our in-house anti-SARS-CoV-2 IgG ELISA displayed a 93.5% sensitivity and 98.8% specificity whereas our in-house anti-SARS-CoV-2 IgA ELISA provided assay sensitivity and specificity at 89.5% and 99.4%, respectively. The agreement kappa values of our in-house anti-SARS-CoV-2 IgG and IgA ELISA assays were deemed to be excellent and fair, respectively, when compared to RT-PCR and excellent for both assays when compared to Euroimmun anti-SARS-CoV-2 IgG and IgA ELISAs. These data indicate that our in-house anti-SARS-CoV-2 IgG and IgA ELISAs are compatible performing assays for the detection of SARS-CoV-2 infection.

Suggested Citation

  • Kamonthip Rungrojcharoenkit & Rungarun Suthangkornkul & Darunee Utennam & Darunee Buddhari & Soontorn Pinpaiboon & Duangrat Mongkolsirichaikul & Stefan Fernandez & Anthony R Jones & Thomas S Cotrone &, 2023. "Standardization of in-house anti-IgG and IgA ELISAs for the detection of COVID-19," PLOS ONE, Public Library of Science, vol. 18(6), pages 1-9, June.
    • Handle: RePEc:plo:pone00:0287107
    DOI: 10.1371/journal.pone.0287107
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