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Predictive value of α-synuclein expression in peripheral blood of multiple sclerosis patients: A two-dimensional assessment of a selected biomarker

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  • Rabat Razia
  • Fazeel Majeed
  • Rehab Amin
  • Shahid Mukhtar
  • Khalid Mahmood
  • Turki Abualait
  • Shahid Bashir
  • Deeba Noreen Baig

Abstract

Introduction: Our study aimed to evaluate whether assessing α-synuclein expression levels in blood samples could provide a reliable and straightforward alternative to existing diagnostic and prognostic methods for neurodegenerative disorders, including multiple sclerosis (MS). We specifically investigated if α-synuclein and IL-6 expression levels from serum and peripheral blood mononuclear cells (PBMCs) could accurately predict MS severity in patients using a two-dimensional approach. Methods: We designed a case-control study to analyze the expression of α-synuclein and IL-6 in the peripheral blood of an MS patient group (n = 51) and a control group (n = 51). We statistically evaluated the PBMCs and serum profiles of α-synuclein and IL-6 in MS patients, along with their age of onset, disease duration, tobacco exposure, and Expanded Disability Status Scale (EDSS) score, using SPSS V22.0 software and GraphPad Prism V9.0. Results: Our findings indicate that α-synuclein production was significantly downregulated in MS patients. Principal component analysis also revealed distinct profiles between MS patients and controls. PBMCs and serum profiles of α-synuclein correlated with the EDSS score, suggesting that disease severity can be predicted using α-synuclein profiles. Moreover, α-synuclein showed a significant correlation with IL-6 and age of onset. Lastly, receiver operating characteristic curves of PBMCs and serum activity of α-synuclein profiles displayed discrimination with area under the curve values of 0.856 and 0.705, respectively. Conclusion: Our results imply that measuring α-synuclein levels in both serum and PBMCs could be a valuable method for diagnosing and predicting MS severity, potentially serving as a non-invasive biomarker for the disease.

Suggested Citation

  • Rabat Razia & Fazeel Majeed & Rehab Amin & Shahid Mukhtar & Khalid Mahmood & Turki Abualait & Shahid Bashir & Deeba Noreen Baig, 2023. "Predictive value of α-synuclein expression in peripheral blood of multiple sclerosis patients: A two-dimensional assessment of a selected biomarker," PLOS ONE, Public Library of Science, vol. 18(8), pages 1-13, August.
    • Handle: RePEc:plo:pone00:0285022
    DOI: 10.1371/journal.pone.0285022
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    1. Paul R. Mangan & Laurie E. Harrington & Darrell B. O'Quinn & Whitney S. Helms & Daniel C. Bullard & Charles O. Elson & Robin D. Hatton & Sharon M. Wahl & Trenton R. Schoeb & Casey T. Weaver, 2006. "Transforming growth factor-β induces development of the TH17 lineage," Nature, Nature, vol. 441(7090), pages 231-234, May.
    2. Estelle Bettelli & Yijun Carrier & Wenda Gao & Thomas Korn & Terry B. Strom & Mohamed Oukka & Howard L. Weiner & Vijay K. Kuchroo, 2006. "Reciprocal developmental pathways for the generation of pathogenic effector TH17 and regulatory T cells," Nature, Nature, vol. 441(7090), pages 235-238, May.
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