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Anomalies in dye-terminator DNA sequencing caused by a natural G-quadruplex

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  • George S Brush

Abstract

A G-rich DNA sequence from yeast that can form a non-canonical G-quadruplex structure was cloned into a plasmid vector and subjected to Sanger sequencing using dye-labeled dideoxynucleotides. Two different effects were observed. In one, presence of the G4 sequence on the template strand led to incorrect incorporation of an A residue at an internal position in the G4 sequence. In the other, the nascent strand caused attenuation of the readout coincident with synthesis of the G-rich DNA. The two effects are novel examples of disruption in DNA synthesis caused by a G4 sequence. These results provide a new example of a DNA structure that could influence genomic stability in human cells.

Suggested Citation

  • George S Brush, 2022. "Anomalies in dye-terminator DNA sequencing caused by a natural G-quadruplex," PLOS ONE, Public Library of Science, vol. 17(12), pages 1-19, December.
    • Handle: RePEc:plo:pone00:0279423
    DOI: 10.1371/journal.pone.0279423
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    1. Paulina Prorok & Marie Artufel & Antoine Aze & Philippe Coulombe & Isabelle Peiffer & Laurent Lacroix & Aurore Guédin & Jean-Louis Mergny & Julia Damaschke & Aloys Schepers & Christelle Cayrou & Marie, 2019. "Involvement of G-quadruplex regions in mammalian replication origin activity," Nature Communications, Nature, vol. 10(1), pages 1-16, December.
    2. Katrin Paeschke & Matthew L. Bochman & P. Daniela Garcia & Petr Cejka & Katherine L. Friedman & Stephen C. Kowalczykowski & Virginia A. Zakian, 2013. "Pif1 family helicases suppress genome instability at G-quadruplex motifs," Nature, Nature, vol. 497(7450), pages 458-462, May.
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