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Cost-efficient boundary-free surface patterning achieves high effective-throughput of time-lapse microscopy experiments

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  • Guohao Liang
  • Hong Yin
  • Jun Allard
  • Fangyuan Ding

Abstract

Time-lapse microscopy plays critical roles in the studies of cellular dynamics. However, setting up a time-lapse movie experiments is not only laborious but also with low output, mainly due to the cell-losing problem (i.e., cells moving out of limited field of view), especially in a long-time recording. To overcome this issue, we have designed a cost-efficient way that enables cell patterning on the imaging surfaces without any physical boundaries. Using mouse embryonic stem cells as an example system, we have demonstrated that our boundary-free patterned surface solves the cell-losing problem without disturbing their cellular phenotype. Statistically, the presented system increases the effective-throughput of time-lapse microscopy experiments by an order of magnitude.

Suggested Citation

  • Guohao Liang & Hong Yin & Jun Allard & Fangyuan Ding, 2022. "Cost-efficient boundary-free surface patterning achieves high effective-throughput of time-lapse microscopy experiments," PLOS ONE, Public Library of Science, vol. 17(10), pages 1-14, October.
    • Handle: RePEc:plo:pone00:0275804
    DOI: 10.1371/journal.pone.0275804
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