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Plasma proteomic analysis to identify potential biomarkers of histologic chorioamnionitis in women with preterm premature rupture of membranes

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  • Ji Eun Lee
  • Kisoon Dan
  • Hyeon Ji Kim
  • Yu Mi Kim
  • Kyo Hoon Park

Abstract

Introduction: To identify potential biomarkers in the plasma that could predict histologic chorioamnionitis (HCA) in women with preterm premature rupture of membranes (PPROM), using shotgun and targeted proteomic analyses. Methods: This retrospective cohort study included 78 singleton pregnant women with PPROM (24–34 gestational weeks) who delivered within 96 h of blood sampling. Maternal plasma samples were analyzed by label-free liquid chromatography-tandem mass spectrometry for proteome profiling in a nested case-control study design (HCA cases vs. non-HCA controls [n = 9 each]). Differential expression of 12 candidate proteins was assessed by multiple reaction monitoring-mass spectrometry (MRM-MS) analysis in individual plasma samples from cases and controls matched by gestational age at sampling (n = 40, cohort 1). A validation study was further performed in an independent study group (n = 38, cohort 2) using ELISA and turbidimetric immunoassay for three differentially expressed proteins. Results: Shotgun proteomics analyses yielded 18 proteins that were differentially expressed (P

Suggested Citation

  • Ji Eun Lee & Kisoon Dan & Hyeon Ji Kim & Yu Mi Kim & Kyo Hoon Park, 2022. "Plasma proteomic analysis to identify potential biomarkers of histologic chorioamnionitis in women with preterm premature rupture of membranes," PLOS ONE, Public Library of Science, vol. 17(7), pages 1-22, July.
    • Handle: RePEc:plo:pone00:0270884
    DOI: 10.1371/journal.pone.0270884
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