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Evaluation of micro-RNA in extracellular vesicles from blood of patients with prostate cancer

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  • Jiyoon Kim
  • Siwoo Cho
  • Yonghyun Park
  • Jiyoul Lee
  • Jaesung Park

Abstract

Extracellular vesicles (EVs) contain various types of molecules including micro-RNAs, so isolating EVs can be an effective way to analyze and diagnose diseases. A lot of micro-RNAs have been known in relation to prostate cancer (PCa), and we evaluate miR-21, miR-141, and miR-221 in EVs and compare them with prostate-specific antigen (PSA). EVs were isolated from plasma of 38 patients with prostate cancer and 8 patients with benign prostatic hyperplasia (BPH), using a method that showed the highest recovery of RNA. Isolation of EVs concentrated micro-RNAs, reducing the cycle threshold (Ct) value of RT-qPCR amplification of micro-RNA such as miR-16 by 5.12 and miR-191 by 4.65, compared to the values before EV isolation. Normalization of target micro-RNAs was done using miR-191. For miR-221, the mean expression level of patients with localized PCa was significantly higher than that of the control group, having 33.45 times higher expression than the control group (p

Suggested Citation

  • Jiyoon Kim & Siwoo Cho & Yonghyun Park & Jiyoul Lee & Jaesung Park, 2021. "Evaluation of micro-RNA in extracellular vesicles from blood of patients with prostate cancer," PLOS ONE, Public Library of Science, vol. 16(12), pages 1-11, December.
    • Handle: RePEc:plo:pone00:0262017
    DOI: 10.1371/journal.pone.0262017
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