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Fast in-vitro screening of FLT3-ITD inhibitors using silkworm-baculovirus protein expression system

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  • Naoki Yamamoto
  • Jiro Kikuchi
  • Yusuke Furukawa
  • Naoya Shibayama

Abstract

We report expression and purification of a FLT3 protein with ITD mutation (FLT3-ITD) with a steady tyrosine kinase activity using a silkworm-baculovirus system, and its application as a fast screening system of tyrosine kinase inhibitors. The FLT3-ITD protein was expressed in Bombyx mori L. pupae infected by gene-modified nucleopolyhedrovirus, and was purified as an active state. We performed an inhibition assay using 17 kinase inhibitors, and succeeded in screening two inhibitors for FLT3-ITD. The result has paved the way for screening FLT3-ITD inhibitors in a fast and easy manner, and also for structural studies.

Suggested Citation

  • Naoki Yamamoto & Jiro Kikuchi & Yusuke Furukawa & Naoya Shibayama, 2022. "Fast in-vitro screening of FLT3-ITD inhibitors using silkworm-baculovirus protein expression system," PLOS ONE, Public Library of Science, vol. 17(5), pages 1-6, May.
    • Handle: RePEc:plo:pone00:0261699
    DOI: 10.1371/journal.pone.0261699
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