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Differentiating iron-loading anemias using a newly developed and analytically validated ELISA for human serum erythroferrone

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  • Laura Diepeveen
  • Rian Roelofs
  • Nicolai Grebenchtchikov
  • Rachel van Swelm
  • Leon Kautz
  • Dorine Swinkels

Abstract

Erythroferrone (ERFE), the erythroid regulator of iron metabolism, inhibits hepcidin to increase iron availability for erythropoiesis. ERFE plays a pathological role during ineffective erythropoiesis as occurs in X-linked sideroblastic anemia (XLSA) and β-thalassemia. Its measurement might serve as an indicator of severity for these diseases. However, for reliable quantification of ERFE analytical characterization is indispensable to determine the assay’s limitations and define proper methodology. We developed a sandwich ELISA for human serum ERFE using polyclonal antibodies and report its extensive analytical validation. This new assay showed, for the first time, the differentiation of XLSA and β-thalassemia major patients from healthy controls (p = 0.03) and from each other (p

Suggested Citation

  • Laura Diepeveen & Rian Roelofs & Nicolai Grebenchtchikov & Rachel van Swelm & Leon Kautz & Dorine Swinkels, 2021. "Differentiating iron-loading anemias using a newly developed and analytically validated ELISA for human serum erythroferrone," PLOS ONE, Public Library of Science, vol. 16(7), pages 1-14, July.
    • Handle: RePEc:plo:pone00:0254851
    DOI: 10.1371/journal.pone.0254851
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