IDEAS home Printed from https://ideas.repec.org/a/plo/pone00/0244712.html
   My bibliography  Save this article

Serological and hematological characteristics of Sjogren’s syndrome and dry eye syndrome patients using a novel immune serology technique

Author

Listed:
  • Hadas Ben-Eli
  • Abraham Solomon
  • Doron J Aframian
  • Eldad Ben-Chetrit
  • Dror Mevorach
  • Geffen Kleinstern
  • Tim Waterboer
  • Martina Willhauck-Fleckenstein
  • Michael Pawlita
  • Ora Paltiel

Abstract

Objectives: To compare hematologic and serological parameters among patients with Sjogren’s syndrome (SS), dry eye syndrome (DES) and controls, and validate a novel multiplex-serology method for identifying auto-antibodies in these populations. Methods: In a clinic-based case-control study a total of 422 participants were recruited, including 91 with SS, 120 DES, and 211 controls (age and sex frequency-matched). We measured blood counts, anti-nuclear-antibodies (ANA), anti-SSA/SSB, anti-ribonucleoprotein (RNP), anti-double-stranded-DNA (DS-DNA), and rheumatoid factor (RF) using the “Immunodot” qualitative-ELISA assay. Immunoglobulins, C3 and C4 were measured by immune-fluorescence. Autoantibodies were also quantified with a newly-developed method using glutathione-S-transferase fusion proteins of SSA/Ro 52 and 60kD and SSB/La (multiplex-serology), measuring median fluorescence intensity (MFI). Results: Among DES patients, only 2% (95%CI: 0.36–6.3) had positive immune serology. SS patients had lower lymphocyte, hemoglobin and C3 levels but higher prevalence of RF, ANA, anti-SSA/B and higher IgG and MFI levels, compared to DES and controls (P

Suggested Citation

  • Hadas Ben-Eli & Abraham Solomon & Doron J Aframian & Eldad Ben-Chetrit & Dror Mevorach & Geffen Kleinstern & Tim Waterboer & Martina Willhauck-Fleckenstein & Michael Pawlita & Ora Paltiel, 2020. "Serological and hematological characteristics of Sjogren’s syndrome and dry eye syndrome patients using a novel immune serology technique," PLOS ONE, Public Library of Science, vol. 15(12), pages 1-13, December.
    • Handle: RePEc:plo:pone00:0244712
    DOI: 10.1371/journal.pone.0244712
    as

    Download full text from publisher

    File URL: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0244712
    Download Restriction: no
    File URL: https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0244712&type=printable
    Download Restriction: no
    File URL: https://libkey.io/10.1371/journal.pone.0244712?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:plo:pone00:0244712. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: plosone (email available below). General contact details of provider: https://journals.plos.org/plosone/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.