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NF-κB-mediated regulation of rat CYP2E1 by two independent signaling pathways

Author

Listed:
  • Qin Lin
  • Xiaolin Kang
  • Xuefeng Li
  • Tao Wang
  • Fengting Liu
  • Jinxue Jia
  • Ziqi Jin
  • Yongzhi Xue

Abstract

Cytochrome P450 2E1 (CYP2E1) plays an important role in both alcohol-induced and immune-mediated liver injury. However, the mechanism underlying CYP2E1 transcriptional regulation has not been clarified. This study focused on the NF-κB-mediated transcriptional regulation of rat CYP2E1 by two independent signaling pathways in alcohol-induced and immune-mediated liver injury rat models. Male Sprague-Dawley rats were used in pharmacokinetic, molecular pharmacology, and morphology experiments. A rat model of alcohol-induced liver injury (AL) was established by feeding an ethanol-containing diet (42 g/kg/day) for 5 weeks as indicated. A rat immune-mediated liver injury (IM) model was established by the sequential injection of bacillus Calmette-Guérin (BCG, 125 mg/kg, once) via the tail vein after test day 21 and 10 μg/kg LPS 13 days later. HPLC, real-time PCR, western blot and ELISA analyses were performed. CYP2E1 expression was enhanced during the process of alcohol-induced liver injury (increased by 56%, P

Suggested Citation

  • Qin Lin & Xiaolin Kang & Xuefeng Li & Tao Wang & Fengting Liu & Jinxue Jia & Ziqi Jin & Yongzhi Xue, 2019. "NF-κB-mediated regulation of rat CYP2E1 by two independent signaling pathways," PLOS ONE, Public Library of Science, vol. 14(12), pages 1-17, December.
    • Handle: RePEc:plo:pone00:0225531
    DOI: 10.1371/journal.pone.0225531
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