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Relative Telomere Repeat Mass in Buccal and Leukocyte-Derived DNA

Author

Listed:
  • Casey T Finnicum
  • Conor V Dolan
  • Gonneke Willemsen
  • Zachary M Weber
  • Jason L Petersen
  • Jeffrey J Beck
  • Veryan Codd
  • Dorret I Boomsma
  • Gareth E Davies
  • Erik A Ehli

Abstract

Telomere length has garnered interest due to the potential role it may play as a biomarker for the cellular aging process. Telomere measurements obtained from blood-derived DNA are often used in epidemiological studies. However, the invasive nature of blood draws severely limits sample collection, particularly with children. Buccal cells are commonly sampled for DNA isolation and thus may present a non-invasive alternative for telomere measurement. Buccal and leukocyte derived DNA obtained from samples collected at the same time period were analyzed for telomere repeat mass (TRM). TRM was measured in buccal-derived DNA samples from individuals for whom previous TRM data from blood samples existed. TRM measurement was performed by qPCR and was normalized to the single copy 36B4 gene relative to a reference DNA sample (K562). Correlations between TRM from blood and buccal DNA were obtained and also between the same blood DNA samples measured in separate laboratories. Using the classical twin design, TRM heritability was estimated (N = 1892, MZ = 1044, DZ = 775). Buccal samples measured for TRM showed a significant correlation with the blood-1 (R = 0.39, p

Suggested Citation

  • Casey T Finnicum & Conor V Dolan & Gonneke Willemsen & Zachary M Weber & Jason L Petersen & Jeffrey J Beck & Veryan Codd & Dorret I Boomsma & Gareth E Davies & Erik A Ehli, 2017. "Relative Telomere Repeat Mass in Buccal and Leukocyte-Derived DNA," PLOS ONE, Public Library of Science, vol. 12(1), pages 1-11, January.
    • Handle: RePEc:plo:pone00:0170765
    DOI: 10.1371/journal.pone.0170765
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