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Prediction of Impending Type 1 Diabetes through Automated Dual-Label Measurement of Proinsulin:C-Peptide Ratio

Author

Listed:
  • Annelien Van Dalem
  • Simke Demeester
  • Eric V Balti
  • Bart Keymeulen
  • Pieter Gillard
  • Bruno Lapauw
  • Christophe De Block
  • Pascale Abrams
  • Eric Weber
  • Ilse Vermeulen
  • Pieter De Pauw
  • Daniël Pipeleers
  • Ilse Weets
  • Frans K Gorus
  • Belgian Diabetes Registry

Abstract

Background: The hyperglycemic clamp test, the gold standard of beta cell function, predicts impending type 1 diabetes in islet autoantibody-positive individuals, but the latter may benefit from less invasive function tests such as the proinsulin:C-peptide ratio (PI:C). The present study aims to optimize precision of PI:C measurements by automating a dual-label trefoil-type time-resolved fluorescence immunoassay (TT-TRFIA), and to compare its diagnostic performance for predicting type 1 diabetes with that of clamp-derived C-peptide release. Methods: Between-day imprecision (n = 20) and split-sample analysis (n = 95) were used to compare TT-TRFIA (AutoDelfia, Perkin-Elmer) with separate methods for proinsulin (in-house TRFIA) and C-peptide (Elecsys, Roche). High-risk multiple autoantibody-positive first-degree relatives (n = 49; age 5–39) were tested for fasting PI:C, HOMA2-IR and hyperglycemic clamp and followed for 20–57 months (interquartile range). Results: TT-TRFIA values for proinsulin, C-peptide and PI:C correlated significantly (r2 = 0.96–0.99; P

Suggested Citation

  • Annelien Van Dalem & Simke Demeester & Eric V Balti & Bart Keymeulen & Pieter Gillard & Bruno Lapauw & Christophe De Block & Pascale Abrams & Eric Weber & Ilse Vermeulen & Pieter De Pauw & Daniël Pipe, 2016. "Prediction of Impending Type 1 Diabetes through Automated Dual-Label Measurement of Proinsulin:C-Peptide Ratio," PLOS ONE, Public Library of Science, vol. 11(12), pages 1-14, December.
    • Handle: RePEc:plo:pone00:0166702
    DOI: 10.1371/journal.pone.0166702
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