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Specificity and Strain-Typing Capabilities of Nanorod Array-Surface Enhanced Raman Spectroscopy for Mycoplasma pneumoniae Detection

Author

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  • Kelley C Henderson
  • Alvaro J Benitez
  • Amy E Ratliff
  • Donna M Crabb
  • Edward S Sheppard
  • Jonas M Winchell
  • Richard A Dluhy
  • Ken B Waites
  • T Prescott Atkinson
  • Duncan C Krause

Abstract

Mycoplasma pneumoniae is a cell wall-less bacterial pathogen of the human respiratory tract that accounts for > 20% of all community-acquired pneumonia (CAP). At present the most effective means for detection and strain-typing is quantitative polymerase chain reaction (qPCR), which can exhibit excellent sensitivity and specificity but requires separate tests for detection and genotyping, lacks standardization between available tests and between labs, and has limited practicality for widespread, point-of-care use. We have developed and previously described a silver nanorod array-surface enhanced Raman Spectroscopy (NA-SERS) biosensing platform capable of detecting M. pneumoniae with statistically significant specificity and sensitivity in simulated and true clinical throat swab samples, and the ability to distinguish between reference strains of the two main genotypes of M. pneumoniae. Furthermore, we have established a qualitative lower endpoint of detection for NA-SERS of

Suggested Citation

  • Kelley C Henderson & Alvaro J Benitez & Amy E Ratliff & Donna M Crabb & Edward S Sheppard & Jonas M Winchell & Richard A Dluhy & Ken B Waites & T Prescott Atkinson & Duncan C Krause, 2015. "Specificity and Strain-Typing Capabilities of Nanorod Array-Surface Enhanced Raman Spectroscopy for Mycoplasma pneumoniae Detection," PLOS ONE, Public Library of Science, vol. 10(6), pages 1-24, June.
    • Handle: RePEc:plo:pone00:0131831
    DOI: 10.1371/journal.pone.0131831
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