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A Novel Approach to Identifying Physical Markers of Cryo-Damage in Bull Spermatozoa

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  • Sung-Jae Yoon
  • Woo-Sung Kwon
  • Md Saidur Rahman
  • June-Sub Lee
  • Myung-Geol Pang

Abstract

Cryopreservation is an efficient way to store spermatozoa and plays a critical role in the livestock industry as well as in clinical practice. During cryopreservation, cryo-stress causes substantial damage to spermatozoa. In present study, the effects of cryo-stress at various cryopreservation steps, such as dilution / cooling, adding cryoprtectant, and freezing were studied in spermatozoa collected from 9 individual bull testes. The motility (%), motion kinematics, capacitation status, mitochondrial activity, and viability of bovine spermatozoa at each step of the cryopreservation process were assessed using computer-assisted sperm analysis, Hoechst 33258/chlortetracycline fluorescence, rhodamine 123 staining, and hypo-osmotic swelling test, respectively. The results demonstrate that the cryopreservation steps reduced motility (%), rapid speed (%), and mitochondrial activity, whereas medium/slow speed (%), and the acrosome reaction were increased (P

Suggested Citation

  • Sung-Jae Yoon & Woo-Sung Kwon & Md Saidur Rahman & June-Sub Lee & Myung-Geol Pang, 2015. "A Novel Approach to Identifying Physical Markers of Cryo-Damage in Bull Spermatozoa," PLOS ONE, Public Library of Science, vol. 10(5), pages 1-12, May.
    • Handle: RePEc:plo:pone00:0126232
    DOI: 10.1371/journal.pone.0126232
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