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Epidermal Growth Factor-Mediated Mitogen-Activated Protein Kinase3/1 Pathway Is Conducive to In Vitro Maturation of Sheep Oocytes

Author

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  • Hemin Ni
  • Xihui Sheng
  • Xu Cui
  • Meichao Gu
  • Yunhai Liu
  • Xiaolong Qi
  • Shuhan Xing
  • Yong Guo

Abstract

Epidermal growth factor (EGF) has been shown to facilitate the in vitro maturation of sheep oocytes, and enhance embryo’s capability for further development. However, such kind of molecular mechanism has not yet been elucidated. In the present study, we investigated the effect of EGF-mediated mitogen-activated protein kinases 3 and 1 (MAPK3/1) pathway on in vitro maturation of sheep oocytes. U0126, a specific inhibitor of MEK (MAPK kinase), was added into the maturation culture medium to block the EGF-mediated MAPK3/1 pathway with different doses. Then, the nuclear maturation of sheep oocytes was examined. Additionally, the effect of EGF-mediated MAPK3/1 on cytoplasmic maturation was examined though in vitro fertilization and embryonic development. The rate of germinal vesicle breakdown (GVBD) after 6 h of culture with 10−4 mol/l of U0126 (50.4%) was significantly decreased compared with control (67.2%, p

Suggested Citation

  • Hemin Ni & Xihui Sheng & Xu Cui & Meichao Gu & Yunhai Liu & Xiaolong Qi & Shuhan Xing & Yong Guo, 2015. "Epidermal Growth Factor-Mediated Mitogen-Activated Protein Kinase3/1 Pathway Is Conducive to In Vitro Maturation of Sheep Oocytes," PLOS ONE, Public Library of Science, vol. 10(3), pages 1-12, March.
    • Handle: RePEc:plo:pone00:0120418
    DOI: 10.1371/journal.pone.0120418
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