IDEAS home Printed from https://ideas.repec.org/a/plo/pone00/0115611.html
   My bibliography  Save this article

Dynamic Growth and Shrinkage Govern the pH Dependence of RecA Filament Stability

Author

Listed:
  • Sung Hyun Kim
  • Jeehae Park
  • Chirlmin Joo
  • Doseok Kim
  • Taekjip Ha

Abstract

RecA proteins form a long stable filament on a single-stranded DNA and catalyze strand exchange reaction. The stability of RecA filament changes dramatically with pH, yet its detailed mechanism is not known. Here, using a single molecule assay, we determined the binding and dissociation rates of RecA monomers at the filament ends at various pH. The pH-induced rate changes were moderate but occurred in opposite directions for binding and dissociation, resulting in a substantial increase in filament stability in lower pH. The highly charged residues in C-terminal domain do not contribute to the pH dependent stability. The stability enhancement of RecA filament in low pH may help the cell to cope with acidic stress by fine-tuning of the binding and dissociation rates without losing the highly dynamic nature of the filament required for strand exchange.

Suggested Citation

  • Sung Hyun Kim & Jeehae Park & Chirlmin Joo & Doseok Kim & Taekjip Ha, 2015. "Dynamic Growth and Shrinkage Govern the pH Dependence of RecA Filament Stability," PLOS ONE, Public Library of Science, vol. 10(1), pages 1-8, January.
    • Handle: RePEc:plo:pone00:0115611
    DOI: 10.1371/journal.pone.0115611
    as

    Download full text from publisher

    File URL: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0115611
    Download Restriction: no
    File URL: https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0115611&type=printable
    Download Restriction: no
    File URL: https://libkey.io/10.1371/journal.pone.0115611?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Roberto Galletto & Ichiro Amitani & Ronald J. Baskin & Stephen C. Kowalczykowski, 2006. "Direct observation of individual RecA filaments assembling on single DNA molecules," Nature, Nature, vol. 443(7113), pages 875-878, October.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.

      More about this item

      Statistics

      Access and download statistics

      Corrections

      All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:plo:pone00:0115611. See general information about how to correct material in RePEc.

      If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

      If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

      If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

      For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: plosone (email available below). General contact details of provider: https://journals.plos.org/plosone/ .

      Please note that corrections may take a couple of weeks to filter through the various RePEc services.

      IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.