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Comparison of Multiple Displacement Amplification (MDA) and Multiple Annealing and Looping-Based Amplification Cycles (MALBAC) in Single-Cell Sequencing

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  • Minfeng Chen
  • Pengfei Song
  • Dan Zou
  • Xuesong Hu
  • Shancen Zhao
  • Shengjie Gao
  • Fei Ling

Abstract

Single-cell sequencing promotes our understanding of the heterogeneity of cellular populations, including the haplotypes and genomic variability among different generation of cells. Whole-genome amplification is crucial to generate sufficient DNA fragments for single-cell sequencing projects. Using sequencing data from single sperms, we quantitatively compare two prevailing amplification methods that extensively applied in single-cell sequencing, multiple displacement amplification (MDA) and multiple annealing and looping-based amplification cycles (MALBAC). Our results show that MALBAC, as a combination of modified MDA and tweaked PCR, has a higher level of uniformity, specificity and reproducibility.

Suggested Citation

  • Minfeng Chen & Pengfei Song & Dan Zou & Xuesong Hu & Shancen Zhao & Shengjie Gao & Fei Ling, 2014. "Comparison of Multiple Displacement Amplification (MDA) and Multiple Annealing and Looping-Based Amplification Cycles (MALBAC) in Single-Cell Sequencing," PLOS ONE, Public Library of Science, vol. 9(12), pages 1-12, December.
    • Handle: RePEc:plo:pone00:0114520
    DOI: 10.1371/journal.pone.0114520
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