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Direct Comparison of Flow-FISH and qPCR as Diagnostic Tests for Telomere Length Measurement in Humans

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  • Fernanda Gutierrez-Rodrigues
  • Bárbara A Santana-Lemos
  • Priscila S Scheucher
  • Raquel M Alves-Paiva
  • Rodrigo T Calado

Abstract

Telomere length measurement is an essential test for the diagnosis of telomeropathies, which are caused by excessive telomere erosion. Commonly used methods are terminal restriction fragment (TRF) analysis by Southern blot, fluorescence in situ hybridization coupled with flow cytometry (flow-FISH), and quantitative PCR (qPCR). Although these methods have been used in the clinic, they have not been comprehensively compared. Here, we directly compared the performance of flow-FISH and qPCR to measure leukocytes' telomere length of healthy individuals and patients evaluated for telomeropathies, using TRF as standard. TRF and flow-FISH showed good agreement and correlation in the analysis of healthy subjects (R2 = 0.60; p

Suggested Citation

  • Fernanda Gutierrez-Rodrigues & Bárbara A Santana-Lemos & Priscila S Scheucher & Raquel M Alves-Paiva & Rodrigo T Calado, 2014. "Direct Comparison of Flow-FISH and qPCR as Diagnostic Tests for Telomere Length Measurement in Humans," PLOS ONE, Public Library of Science, vol. 9(11), pages 1-9, November.
    • Handle: RePEc:plo:pone00:0113747
    DOI: 10.1371/journal.pone.0113747
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