IDEAS home Printed from https://ideas.repec.org/a/plo/pone00/0109914.html
   My bibliography  Save this article

Optimized Method for Preparation of IgG-Binding Bacterial Magnetic Nanoparticles

Author

Listed:
  • Denis S Grouzdev
  • Marina V Dziuba
  • Denis V Kurek
  • Alexander I Ovchinnikov
  • Nadezhda A Zhigalova
  • Boris B Kuznetsov
  • Konstantin G Skryabin

Abstract

In this study, the optimized method for designing IgG-binding magnetosomes based on integration of IgG-binding fusion proteins into magnetosome membrane in vitro is presented. Fusion proteins Mbb and Mistbb consisting of magnetosome membrane protein MamC and membrane associating protein Mistic from Bacillus subtilis as anchors and BB-domains of Staphylococcus aureus protein A as IgG-binding region were used. With Response Surface Methodology (RSM) the highest level of proteins integration into magnetosome membrane was achieved under the following parameters: pH 8.78, without adding NaCl and 55 s of vortexing for Mbb; pH 9.48, 323 mM NaCl and 55 s of vortexing for Mistbb. Modified magnetosomes with Mbb and Mistbb displayed on their surface demonstrated comparable levels of IgG-binding activity, suggesting that both proteins could be efficiently used as anchor molecules. We also demonstrated that such modified magnetosomes are stable in PBS buffer during at least two weeks. IgG-binding magnetosomes obtained by this approach could serve as a multifunctional platform for displaying various types of antibodies.

Suggested Citation

  • Denis S Grouzdev & Marina V Dziuba & Denis V Kurek & Alexander I Ovchinnikov & Nadezhda A Zhigalova & Boris B Kuznetsov & Konstantin G Skryabin, 2014. "Optimized Method for Preparation of IgG-Binding Bacterial Magnetic Nanoparticles," PLOS ONE, Public Library of Science, vol. 9(10), pages 1-15, October.
    • Handle: RePEc:plo:pone00:0109914
    DOI: 10.1371/journal.pone.0109914
    as

    Download full text from publisher

    File URL: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0109914
    Download Restriction: no
    File URL: https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0109914&type=printable
    Download Restriction: no
    File URL: https://libkey.io/10.1371/journal.pone.0109914?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:plo:pone00:0109914. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: plosone (email available below). General contact details of provider: https://journals.plos.org/plosone/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.