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Effects of siRNA on RET/PTC3 Junction Oncogene in Papillary Thyroid Carcinoma: From Molecular and Cellular Studies to Preclinical Investigations

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  • Hafiz Muhammad Ali
  • Giorgia Urbinati
  • Hubert Chapuis
  • Didier DesmaEle
  • Jean-Rémi Bertrand
  • Patrick Couvreur
  • Liliane Massaad-Massade

Abstract

RET/PTC3 junction oncogene is typical of radiation-induced childhood papillary thyroid carcinoma (PTC) with a short latency period. Since, RET/PTC3 is only present in the tumour cells, thus represents an interesting target for specific therapy by small interfering RNA (siRNA). Our aim is to demonstrate in vitro and in vivo molecular and cellular effects of siRNA on RET/PTC3 knockdown for therapeutic application.First, we established a novel cell line stably expressing RET/PTC3 junction oncogene, named RP3 which was found tumorigenic in nude mice compared to NIH/3T3 mouse fibroblasts. Among four siRNAs and five concentrations tested against RET/PTC3, an efficient siRNA RET/PTC3 and an appropriate dose (50 nM) were selected which showed significant inhibition (p

Suggested Citation

  • Hafiz Muhammad Ali & Giorgia Urbinati & Hubert Chapuis & Didier DesmaEle & Jean-Rémi Bertrand & Patrick Couvreur & Liliane Massaad-Massade, 2014. "Effects of siRNA on RET/PTC3 Junction Oncogene in Papillary Thyroid Carcinoma: From Molecular and Cellular Studies to Preclinical Investigations," PLOS ONE, Public Library of Science, vol. 9(4), pages 1-12, April.
    • Handle: RePEc:plo:pone00:0095964
    DOI: 10.1371/journal.pone.0095964
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