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Determination and Modulation of Total and Surface Calcium-Sensing Receptor Expression in Monocytes In Vivo and In Vitro

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Listed:
  • Julien Paccou
  • Cédric Boudot
  • Aurélien Mary
  • Said Kamel
  • Tilman Bernhard Drüeke
  • Patrice Fardellone
  • Ziad Massy
  • Michel Brazier
  • Romuald Mentaverri

Abstract

Expression of the calcium-sensing receptor (CaSR) has previously been demonstrated in human circulating monocytes (HCM). The present study was designed to measure CaSR expression in HCM and to examine its potential modulation by pro-inflammatory cytokines, Ca2+, vitamin D sterols in U937 cell line. Twenty healthy volunteers underwent blood sampling with subsequent isolation of peripheral blood mononuclear cells (PBMC) at 3 visits. Flow cytometry analysis (FACS) was performed initially (V1) and 19 days later (V2) to examine intra- and intersubject fluctuations of total and surface CaSR expression in HCM and 15 weeks later (V3) to study the effect of vitamin D supplementation. In vitro experiments were conducted to assess the effects of pro-inflammatory cytokines, calcidiol, calcitriol and Ca2+ on CaSR expression in U937 cell line. By FACS analysis, more than 95% of HCM exhibited cell surface CaSR staining. In contrast, CaSR staining failed to detect surface CaSR expression in other PBMC. After cell permeabilization, total CaSR expression was observed in more than 95% of all types of PBMC. Both total and surface CaSR expression in HCM showed a high degree of intra-assay reproducibility (

Suggested Citation

  • Julien Paccou & Cédric Boudot & Aurélien Mary & Said Kamel & Tilman Bernhard Drüeke & Patrice Fardellone & Ziad Massy & Michel Brazier & Romuald Mentaverri, 2013. "Determination and Modulation of Total and Surface Calcium-Sensing Receptor Expression in Monocytes In Vivo and In Vitro," PLOS ONE, Public Library of Science, vol. 8(10), pages 1-8, October.
    • Handle: RePEc:plo:pone00:0074800
    DOI: 10.1371/journal.pone.0074800
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