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Optimization of Fermentation Conditions and Properties of an Exopolysaccharide from Klebsiella sp. H-207 and Application in Adsorption of Hexavalent Chromium

Author

Listed:
  • Li Qiang
  • Li Yumei
  • Han Sheng
  • Liu Yingzi
  • Song Dongxue
  • Hao Dake
  • Wang Jiajia
  • Qu Yanhong
  • Zheng Yuxia

Abstract

The novel exopolysaccharide HZ-7 is produced by Klebsiella sp. H-207, and its fermentation conditions were optimized by response surface methodology (RSM). In this study, the optimized medium consisted of sucrose 31.93 g/L, KNO3 2.17 g/L and K2HPO4 5.47 g/L; while the optimized culture conditions consisted of seed age 13 h, with an inoculum size of 10.6% and incubation temperature of 28.9°C. A maximum HZ-7 yield of about 15.05 g/L was achieved under the optimized conditions using RSM and single-factor experiments. Next the exopolysaccharide HZ-7 was partially purified and characterized. The resulting product showed good properties, such as high concentration of uronic acid (41.67%), low average molecular weight (about 1.94×105 Da) and porous surface structure, were very advantageous to biosorption. Therefore HZ-7 was applied to absorb hexavalent chromium (Cr(VI)). The maximum adsorption efficiency (99.2%) which was obtained at an initial pH of 1.0 along with an initial Cr(VI) concentration of 20 mg/L, was not affected by ordinary metal ions and temperature. These data suggest Klebsiella sp. H-207 exopolysaccharide will be promising potential for industrial application.

Suggested Citation

  • Li Qiang & Li Yumei & Han Sheng & Liu Yingzi & Song Dongxue & Hao Dake & Wang Jiajia & Qu Yanhong & Zheng Yuxia, 2013. "Optimization of Fermentation Conditions and Properties of an Exopolysaccharide from Klebsiella sp. H-207 and Application in Adsorption of Hexavalent Chromium," PLOS ONE, Public Library of Science, vol. 8(1), pages 1-11, January.
  • Handle: RePEc:plo:pone00:0053542
    DOI: 10.1371/journal.pone.0053542
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