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Age-Associated Changes In Oxidative Stress and NAD+ Metabolism In Human Tissue

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  • Hassina Massudi
  • Ross Grant
  • Nady Braidy
  • Jade Guest
  • Bruce Farnsworth
  • Gilles J Guillemin

Abstract

Nicotinamide adenine dinucleotide (NAD+) is an essential electron transporter in mitochondrial respiration and oxidative phosphorylation. In genomic DNA, NAD+ also represents the sole substrate for the nuclear repair enzyme, poly(ADP-ribose) polymerase (PARP) and the sirtuin family of NAD-dependent histone deacetylases. Age associated increases in oxidative nuclear damage have been associated with PARP-mediated NAD+ depletion and loss of SIRT1 activity in rodents. In this study, we further investigated whether these same associations were present in aging human tissue. Human pelvic skin samples were obtained from consenting patients aged between 15–77 and newborn babies (0–1 year old) (n = 49) previously scheduled for an unrelated surgical procedure. DNA damage correlated strongly with age in both males (p = 0.029; r = 0.490) and females (p = 0.003; r = 0.600) whereas lipid oxidation (MDA) levels increased with age in males (p = 0.004; r = 0.623) but not females (p = 0.3734; r = 0.200). PARP activity significantly increased with age in males (p

Suggested Citation

  • Hassina Massudi & Ross Grant & Nady Braidy & Jade Guest & Bruce Farnsworth & Gilles J Guillemin, 2012. "Age-Associated Changes In Oxidative Stress and NAD+ Metabolism In Human Tissue," PLOS ONE, Public Library of Science, vol. 7(7), pages 1-9, July.
    • Handle: RePEc:plo:pone00:0042357
    DOI: 10.1371/journal.pone.0042357
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