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Quantitative Image Analysis and Modeling Indicate the Agrobacterium tumefaciens Type IV Secretion System Is Organized in a Periodic Pattern of Foci

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  • Todd A Cameron
  • Marcus Roper
  • Patricia C Zambryski

Abstract

The Gram negative plant pathogen Agrobacterium tumefaciens is uniquely capable of genetically transforming eukaryotic host cells during the infection process. DNA and protein substrates are transferred into plant cells via a type IV secretion system (T4SS), which forms large cell-envelope spanning complexes at multiple sites around the bacterial circumference. To gain a detailed understanding of T4SS positioning, the spatial distribution of fluorescently labeled T4SS components was quantitatively assessed to distinguish between random and structured localization processes. Through deconvolution microscopy followed by Fourier analysis and modeling, T4SS foci were found to localize in a non-random periodic pattern. These results indicate that T4SS complexes are dependent on an underlying scaffold or assembly process to obtain an organized distribution suitable for effective delivery of substrates into host cells.

Suggested Citation

  • Todd A Cameron & Marcus Roper & Patricia C Zambryski, 2012. "Quantitative Image Analysis and Modeling Indicate the Agrobacterium tumefaciens Type IV Secretion System Is Organized in a Periodic Pattern of Foci," PLOS ONE, Public Library of Science, vol. 7(7), pages 1-7, July.
    • Handle: RePEc:plo:pone00:0042219
    DOI: 10.1371/journal.pone.0042219
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    References listed on IDEAS

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    1. Vidya Chandran & Rémi Fronzes & Stéphane Duquerroy & Nora Cronin & Jorge Navaza & Gabriel Waksman, 2009. "Structure of the outer membrane complex of a type IV secretion system," Nature, Nature, vol. 462(7276), pages 1011-1015, December.
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