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Combined Multivariate and Pathway Analyses Show That Allergen-Induced Gene Expression Changes in CD4+ T Cells Are Reversed by Glucocorticoids

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  • Yelin Zhao
  • Hui Wang
  • Mika Gustafsson
  • Antonella Muraro
  • Sören Bruhn
  • Mikael Benson

Abstract

Background: Glucocorticoids (GCs) play a key role in the treatment of allergy. However, the genome-wide effects of GCs on gene expression in allergen-challenged CD4+ T cells have not been described. The aim of this study was to perform a genome-wide analysis to investigate whether allergen-induced gene expression changes in CD4+ T cells could be reversed by GCs. Methodology/Principal Findings: Gene expression microarray analysis was performed to profile gene expression in diluent- (D), allergen- (A), and allergen + hydrocortisone- (T) challenged CD4+ T cells from patients with seasonal allergic rhinitis. Principal component analysis (PCA) showed good separation of the three groups. To identify the correlation between changes in gene expression in allergen-challenged CD4+ T cells before and after GC treatment, we performed orthogonal partial least squares discriminant analysis (OPLS-DA) followed by Pearson correlation analysis. This revealed that allergen-induced genes were widely reversed by GC treatment (r = −0.77, P

Suggested Citation

  • Yelin Zhao & Hui Wang & Mika Gustafsson & Antonella Muraro & Sören Bruhn & Mikael Benson, 2012. "Combined Multivariate and Pathway Analyses Show That Allergen-Induced Gene Expression Changes in CD4+ T Cells Are Reversed by Glucocorticoids," PLOS ONE, Public Library of Science, vol. 7(6), pages 1-7, June.
    • Handle: RePEc:plo:pone00:0039016
    DOI: 10.1371/journal.pone.0039016
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    1. Hui Wang & Johan Gottfries & Fredrik Barrenäs & Mikael Benson, 2011. "Identification of Novel Biomarkers in Seasonal Allergic Rhinitis by Combining Proteomic, Multivariate and Pathway Analysis," PLOS ONE, Public Library of Science, vol. 6(8), pages 1-8, August.
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