IDEAS home Printed from https://ideas.repec.org/a/plo/pone00/0036988.html
   My bibliography  Save this article

PtdIns (3,4,5) P3 Recruitment of Myo10 Is Essential for Axon Development

Author

Listed:
  • Huali Yu
  • Nannan Wang
  • Xingda Ju
  • Yan Yang
  • Dong Sun
  • Mingming Lai
  • Lei Cui
  • Muhammad Abid Sheikh
  • Jianhua Zhang
  • Xingzhi Wang
  • Xiaojuan Zhu

Abstract

Myosin X (Myo10) with pleckstrin homology (PH) domains is a motor protein acting in filopodium initiation and extension. However, its potential role has not been fully understood, especially in neuronal development. In the present study the preferential accumulation of Myo10 in axon tips has been revealed in primary culture of hippocampal neurons with the aid of immunofluorescence from anti-Myo10 antibody in combination with anti-Tuj1 antibody as specific marker. Knocking down Myo10 gene transcription impaired outgrowth of axon with loss of Tau-1-positive phenotype. Interestingly, inhibition of actin polymerization by cytochalasin D rescued the defect of axon outgrowth. Furthermore, ectopic expression of Myo10 with enhanced green fluorescence protein (EGFP) labeled Myo10 mutants induced multiple axon-like neurites in a motor-independent way. Mechanism studies demonstrated that the recruitment of Myo10 through its PH domain to phosphatidylinositol (3,4,5)-trisphosphate (PtdIns (3,4,5) P3) was essential for axon formation. In addition, in vivo studies confirmed that Myo10 was required for neuronal morphological transition during radial neuronal migration in the developmental neocortex.

Suggested Citation

  • Huali Yu & Nannan Wang & Xingda Ju & Yan Yang & Dong Sun & Mingming Lai & Lei Cui & Muhammad Abid Sheikh & Jianhua Zhang & Xingzhi Wang & Xiaojuan Zhu, 2012. "PtdIns (3,4,5) P3 Recruitment of Myo10 Is Essential for Axon Development," PLOS ONE, Public Library of Science, vol. 7(5), pages 1-11, May.
    • Handle: RePEc:plo:pone00:0036988
    DOI: 10.1371/journal.pone.0036988
    as

    Download full text from publisher

    File URL: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0036988
    Download Restriction: no
    File URL: https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0036988&type=printable
    Download Restriction: no
    File URL: https://libkey.io/10.1371/journal.pone.0036988?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:plo:pone00:0036988. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: plosone (email available below). General contact details of provider: https://journals.plos.org/plosone/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.