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Identification of Mouse Serum miRNA Endogenous References by Global Gene Expression Profiles

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  • Qing-Sheng Mi
  • Matthew Weiland
  • Rui-Qun Qi
  • Xing-Hua Gao
  • Laila M Poisson
  • Li Zhou

Abstract

MicroRNAs (miRNAs) are recently discovered small non-coding RNAs and can serve as serum biomarkers for disease diagnosis and prognoses. Lack of reliable serum miRNA endogenous references for normalization in miRNA gene expression makes single miRNA assays inaccurate. Using TaqMan® real-time PCR miRNA arrays with a global gene expression normalization strategy, we have analyzed serum miRNA expression profiles of 20 female mice of NOD/ShiLtJ (n = 8), NOR/LtJ (n = 6), and C57BL/6J (n = 6) at different ages and disease conditions. We identified five miRNAs, miR-146a, miR-16, miR-195, miR-30e and miR-744, to be stably expressed in all strains, which could serve as mouse serum miRNA endogenous references for single assay experiments.

Suggested Citation

  • Qing-Sheng Mi & Matthew Weiland & Rui-Qun Qi & Xing-Hua Gao & Laila M Poisson & Li Zhou, 2012. "Identification of Mouse Serum miRNA Endogenous References by Global Gene Expression Profiles," PLOS ONE, Public Library of Science, vol. 7(2), pages 1-6, February.
    • Handle: RePEc:plo:pone00:0031278
    DOI: 10.1371/journal.pone.0031278
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