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Three-Dimensional X-ray Observation of Atmospheric Biological Samples by Linear-Array Scanning-Electron Generation X-ray Microscope System

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  • Toshihiko Ogura

Abstract

Recently, we developed a soft X-ray microscope called the scanning-electron generation X-ray microscope (SGXM), which consists of a simple X-ray detection system that detects X-rays emitted from the interaction between a scanning electron beam (EB) and the thin film of the sample mount. We present herein a three-dimensional (3D) X-ray detection system that is based on the SGXM technology and designed for studying atmospheric biological samples. This 3D X-ray detection system contains a linear X-ray photodiode (PD) array. The specimens are placed under a CuZn-coated Si3N4 thin film, which is attached to an atmospheric sample holder. Multiple tilt X-ray images of the samples are detected simultaneously by the linear array of X-ray PDs, and the 3D structure is calculated by a new 3D reconstruction method that uses a simulated-annealing algorithm. The resulting 3D models clearly reveal the inner structure of the bacterium. In addition, the proposed method can easily be used for diverse samples in a broad range of scientific fields.

Suggested Citation

  • Toshihiko Ogura, 2011. "Three-Dimensional X-ray Observation of Atmospheric Biological Samples by Linear-Array Scanning-Electron Generation X-ray Microscope System," PLOS ONE, Public Library of Science, vol. 6(6), pages 1-9, June.
    • Handle: RePEc:plo:pone00:0021516
    DOI: 10.1371/journal.pone.0021516
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    1. Andrej Sali & Robert Glaeser & Thomas Earnest & Wolfgang Baumeister, 2003. "From words to literature in structural proteomics," Nature, Nature, vol. 422(6928), pages 216-225, March.
    2. Jannik C. Meyer & C. O. Girit & M. F. Crommie & A. Zettl, 2008. "Imaging and dynamics of light atoms and molecules on graphene," Nature, Nature, vol. 454(7202), pages 319-322, July.
    3. Emiel de Smit & Ingmar Swart & J. Fredrik Creemer & Gerard H. Hoveling & Mary K. Gilles & Tolek Tyliszczak & Patricia J. Kooyman & Henny W. Zandbergen & Cynthia Morin & Bert M. Weckhuysen & Frank M. F, 2008. "Nanoscale chemical imaging of a working catalyst by scanning transmission X-ray microscopy," Nature, Nature, vol. 456(7219), pages 222-225, November.
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