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Growth-Rate Dependence Reveals Design Principles of Plasmid Copy Number Control

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  • Stefan Klumpp

Abstract

Genetic circuits in bacteria are intimately coupled to the cellular growth rate as many parameters of gene expression are growth-rate dependent. Growth-rate dependence can be particularly pronounced for genes on plasmids; therefore the native regulatory systems of a plasmid such as its replication control system are characterized by growth-rate dependent parameters and regulator concentrations. This natural growth-rate dependent variation of regulator concentrations can be used for a quantitative analysis of the design of such regulatory systems. Here we analyze the growth-rate dependence of parameters of the copy number control system of ColE1-type plasmids in E. coli. This analysis allows us to infer the form of the control function and suggests that the Rom protein increases the sensitivity of control.

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  • Stefan Klumpp, 2011. "Growth-Rate Dependence Reveals Design Principles of Plasmid Copy Number Control," PLOS ONE, Public Library of Science, vol. 6(5), pages 1-6, May.
    • Handle: RePEc:plo:pone00:0020403
    DOI: 10.1371/journal.pone.0020403
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