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A Molecular Score by Quantitative PCR as a New Prognostic Tool at Diagnosis for Chronic Lymphocytic Leukemia Patients

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Listed:
  • Basile Stamatopoulos
  • Nathalie Meuleman
  • Cécile De Bruyn
  • Karlien Pieters
  • Géraldine Anthoine
  • Philippe Mineur
  • Dominique Bron
  • Laurence Lagneaux

Abstract

Background: Several markers have been proposed to predict the outcome of chronic lymphocytic leukemia (CLL) patients. However, discordances exist between the current prognostic factors, indicating that none of these factors are totally perfect. Methodology/Principal Findings: Here, we compared the prognostic power of new RNA-based markers in order to construct a quantitative PCR (qPCR) score composed of the most powerful factors. ZAP70, LPL, CLLU1, microRNA-29c and microRNA-223 were measured by real time PCR in a cohort of 170 patients with a median follow-up of 64 months (range3-330). For each patient, cells were obtained at diagnosis and RNA was extracted from purified CD19 cells. The best markers were included in a qPCR score, which was thereafter compared to each individual factor. Statistical analysis showed that all five RNA-based markers can predict treatment-free survival (TFS), but only ZAP70, LPL and microRNA-29c could significantly predict overall survival (OS). These three markers were thus included in a simple qPCR score that was able to significantly predict TFS and OS by dividing patients into three groups (0/3, 1-2/3 and 3/3). Median TFS were >210, 61 and 24 months (P 330, 242 and 137 months (P

Suggested Citation

  • Basile Stamatopoulos & Nathalie Meuleman & Cécile De Bruyn & Karlien Pieters & Géraldine Anthoine & Philippe Mineur & Dominique Bron & Laurence Lagneaux, 2010. "A Molecular Score by Quantitative PCR as a New Prognostic Tool at Diagnosis for Chronic Lymphocytic Leukemia Patients," PLOS ONE, Public Library of Science, vol. 5(9), pages 1-12, September.
    • Handle: RePEc:plo:pone00:0012780
    DOI: 10.1371/journal.pone.0012780
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