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High-Content, Image-Based Screening for Drug Targets in Yeast

Author

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  • Shinsuke Ohnuki
  • Satomi Oka
  • Satoru Nogami
  • Yoshikazu Ohya

Abstract

Background: Drug discovery and development are predicated on elucidation of the potential mechanisms of action and cellular targets of candidate chemical compounds. Recent advances in high-content imaging techniques allow simultaneous analysis of a range of cellular events. In this study, we propose a novel strategy to identify drug targets by combining genetic screening and high-content imaging in yeast. Methodology: In this approach, we infer the cellular functions affected by candidate drugs by comparing morphologic changes induced by the compounds with the phenotypes of yeast mutants. Conclusions: Using this method and four well-characterized reagents, we successfully identified previously known target genes of the compounds as well as other genes involved with functionally related cellular pathways. This is the first demonstration of a genetic high-content assay that can be used to identify drug targets based on morphologic phenotypes of a reference mutant panel.

Suggested Citation

  • Shinsuke Ohnuki & Satomi Oka & Satoru Nogami & Yoshikazu Ohya, 2010. "High-Content, Image-Based Screening for Drug Targets in Yeast," PLOS ONE, Public Library of Science, vol. 5(4), pages 1-11, April.
    • Handle: RePEc:plo:pone00:0010177
    DOI: 10.1371/journal.pone.0010177
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