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Two Forms of Activation-Induced Cytidine Deaminase Differing in Their Ability to Bind Agarose

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  • Mirjam Metzner
  • Wolfgang Schuh
  • Edith Roth
  • Hans-Martin Jäck
  • Matthias Wabl

Abstract

Background: Activation-induced cytidine deaminase (AID) is a B-cell-specific DNA mutator that plays a key role in the formation of the secondary antibody repertoire in germinal center B cells. In the search for binding partners, protein coimmunoprecipitation assays are often performed, generally with agarose beads. Methodology/Principal Findings: We found that, regardless of whether cell lysates containing exogenous or endogenous AID were examined, one of two mouse AID forms bound to agarose alone. Conclusions/Significance: These binding characteristics may be due to the known post-translational modifications of AID; they may also need to be considered in coimmunoprecipitation experiments to avoid false-positive results.

Suggested Citation

  • Mirjam Metzner & Wolfgang Schuh & Edith Roth & Hans-Martin Jäck & Matthias Wabl, 2010. "Two Forms of Activation-Induced Cytidine Deaminase Differing in Their Ability to Bind Agarose," PLOS ONE, Public Library of Science, vol. 5(1), pages 1-6, January.
    • Handle: RePEc:plo:pone00:0008883
    DOI: 10.1371/journal.pone.0008883
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