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Field validation and application of the luminex triplex HIV assay to estimate HIV prevalence and HIV-1 incidence in Nigeria

Author

Listed:
  • Ernest L Yufenyuy
  • Olusola A Akanbi
  • Vedapuri Shanmugam
  • Kelsie Decker-Pulice
  • Jeni Vuong
  • Mervi Detorio
  • Amy Zheng
  • Orji Bassey
  • Ado G Abubakar
  • Oluwaseun Akinmulero
  • Mudiaga Esiekpe
  • Andrew Thomas
  • Iliyasu Abubakar Bichi
  • Israel Tamunonengiyeofori
  • Chinwe Ugwu
  • Evbuomwan Erasogie
  • William Nwachukwu
  • Nwando Mba
  • Ndidi Agala
  • Megan Bronson
  • Hetal K Patel
  • Nnaemeka C Iriemenem
  • Stacie Greby
  • McPaul I Okoye
  • Mahesh Swaminathan
  • Bharat S Parekh
  • Chikwe Ihekweazu

Abstract

HIV cross-sectional surveys require multi-layered testing with several tests to estimate HIV prevalence and HIV-1 incidence. We evaluated the performance and accuracy of the newly developed HIV Triplex assay to diagnose HIV-1 and HIV-2 and detect HIV-1 recent infections using plasma samples from the 2018 Nigeria AIDS Indicator and Impact Survey (NAIIS). Plasma samples from consenting HIV-positive (n=2,773) and a subset of HIV-negative samples (n=7,196), as determined by the national rapid testing algorithm, followed by Bio-Rad Geenius HIV-1/2 Supplemental Assay and Western Blot, aged 18 months - 64 years, were tested using the Luminex-based HIV Triplex assay. The assay classified specimens as HIV-1 positive, HIV-2 positive, dual (HIV-1 & 2) infections, or HIV-seronegative. All HIV-1 and dual infections were further classified as either HIV-1 recent (

Suggested Citation

  • Ernest L Yufenyuy & Olusola A Akanbi & Vedapuri Shanmugam & Kelsie Decker-Pulice & Jeni Vuong & Mervi Detorio & Amy Zheng & Orji Bassey & Ado G Abubakar & Oluwaseun Akinmulero & Mudiaga Esiekpe & Andr, 2025. "Field validation and application of the luminex triplex HIV assay to estimate HIV prevalence and HIV-1 incidence in Nigeria," PLOS Global Public Health, Public Library of Science, vol. 5(4), pages 1-13, April.
    • Handle: RePEc:plo:pgph00:0003455
    DOI: 10.1371/journal.pgph.0003455
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