Stimulus presentation can enhance spiking irregularity across subcortical and cortical regions

Stimulus presentation is believed to quench neural response variability as measured by fano-factor (FF). However, the relative contributions of within-trial spike irregularity and trial-to-trial rate variability to FF fluctuations have remained elusive. Here, we introduce a principled approach for accurate estimation of spiking irregularity and rate variability in time for doubly stochastic point processes. Consistent with previous evidence, analysis showed stimulus-induced reduction in rate variability across multiple cortical and subcortical areas. However, unlike what was previously thought, spiking irregularity, was not constant in time but could be enhanced due to factors such as bursting abating the quench in the post-stimulus FF. Simulations confirmed plausibility of a time varying spiking irregularity arising from within and between pool correlations of excitatory and inhibitory neural inputs. By accurate parsing of neural variability, our approach reveals previously unnoticed changes in neural response variability and constrains candidate mechanisms that give rise to observed rate variability and spiking irregularity within brain regions.Author summary: Mounting evidence suggest neural response variability to be important for understanding and constraining the underlying neural mechanisms in a given brain area. Here, by analyzing responses across multiple brain areas and by using a principled method for parsing variability components into rate variability and spiking irregularity, we show that unlike what was previously thought, event-related quench of variability is not a brain-wide phenomenon and that point process variability and nonrenewal bursting can enhance post-stimulus spiking irregularity across certain cortical and subcortical regions. We propose possible presynaptic mechanisms that may underlie the observed heterogeneities in spiking variability across the brain.