A Pilot Study of Methods about Detection of Inter-Molecular Interactions as Individual Units of Intra-Cellular Structures, Intra- And Extra-Cellular Cascade Regulatory Pathways

Different methods for investigation on the mechanisms of participation of cell proteins in direct and/or indirect intra- and extra-cellular inter-molecular (protein-lipid, protein-protein, protein-RNA, protein-DNA interactions, etc.), by cascade regulatory pathways were developed and tested. These bio-molecules (in particular microtubule proteins and cyclins) have been found as parts from different complex structures from the nuclear, cytoskeleton and membrane cell fractions, as mitotic spindle, endocytosis vesicles, cell organelles, membrane structures, which are important for basic cell functions. The techniques, developed in the current study, could be useful for identification of the proteins/peptides, participating in the composition of these structures, but also of the interactions between them, on molecular and intra-molecular levels. For confirmation of the results obtained and the reliability of the applied methods, routine techniques as scanning electron microscopy (SEM) and transmission electron microscopy (TEM) assays were used. In all cases, the main goal is directed to search of molecules, participating in the control of cell growth and proliferation, as well as of such, which determine the further cell fate during cell differentiation and maturation.