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A framework for neural organoids, assembloids and transplantation studies

Author

Listed:
  • Sergiu P. Pașca

    (Stanford University
    Stanford University)

  • Paola Arlotta

    (Harvard University
    Broad Institute of MIT and Harvard)

  • Helen S. Bateup

    (University of California Berkeley
    University of California Berkeley)

  • J. Gray Camp

    (Roche Innovation Center Basel
    University of Basel)

  • Silvia Cappello

    (Ludwig Maximilian University of Munich
    Max Planck Institute of Psychiatry)

  • Fred H. Gage

    (The Salk Institute for Biological Studies)

  • Jürgen A. Knoblich

    (Vienna Biocenter
    Medical University of Vienna)

  • Arnold R. Kriegstein

    (University of California San Francisco
    University of California San Francisco)

  • Madeline A. Lancaster

    (MRC Laboratory of Molecular Biology
    University of Cambridge)

  • Guo-Li Ming

    (University of Pennsylvania
    University of Pennsylvania
    University of Pennsylvania
    University of Pennsylvania)

  • Gaia Novarino

    (Institute of Science and Technology of Austria)

  • Hideyuki Okano

    (Keio University Regenerative Medicine Research Center)

  • Malin Parmar

    (Lund University)

  • In-Hyun Park

    (Yale School of Medicine
    Yale University)

  • Orly Reiner

    (Weizmann Institute of Science
    Weizmann Institute of Science)

  • Hongjun Song

    (University of Pennsylvania
    University of Pennsylvania
    University of Pennsylvania
    University of Pennsylvania)

  • Lorenz Studer

    (Sloan Kettering Institute for Cancer Research)

  • Jun Takahashi

    (Kyoto University)

  • Sally Temple

    (Neural Stem Cell Institute)

  • Giuseppe Testa

    (University of Milan
    Viale Rita Levi Montalcini)

  • Barbara Treutlein

    (ETH Zürich)

  • Flora M. Vaccarino

    (Yale University
    Yale University
    Yale University
    Yale Kavli Institute for Neuroscience)

  • Pierre Vanderhaeghen

    (VIB-KU Leuven Center for Brain and Disease Research
    Leuven Brain Institute)

  • Tracy Young-Pearse

    (Brigham and Women’s Hospital and Harvard Medical School
    Harvard University)

Abstract

As the field of neural organoids and assembloids expands, there is an emergent need for guidance and advice on designing, conducting and reporting experiments to increase the reproducibility and utility of these models. In this Perspective, we present a framework for the experimental process that encompasses ensuring the quality and integrity of human pluripotent stem cells, characterizing and manipulating neural cells in vitro, transplantation techniques and considerations for modelling human development, evolution and disease. As with all scientific endeavours, we advocate for rigorous experimental designs tailored to explicit scientific questions as well as transparent methodologies and data sharing to provide useful knowledge for current research practices and for developing regulatory standards.

Suggested Citation

  • Sergiu P. Pașca & Paola Arlotta & Helen S. Bateup & J. Gray Camp & Silvia Cappello & Fred H. Gage & Jürgen A. Knoblich & Arnold R. Kriegstein & Madeline A. Lancaster & Guo-Li Ming & Gaia Novarino & Hi, 2025. "A framework for neural organoids, assembloids and transplantation studies," Nature, Nature, vol. 639(8054), pages 315-320, March.
    • Handle: RePEc:nat:nature:v:639:y:2025:i:8054:d:10.1038_s41586-024-08487-6
    DOI: 10.1038/s41586-024-08487-6
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