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Enhanced proofreading governs CRISPR–Cas9 targeting accuracy

Author

Listed:
  • Janice S. Chen

    (University of California)

  • Yavuz S. Dagdas

    (Biophysics Graduate Group, University of California)

  • Benjamin P. Kleinstiver

    (Molecular Pathology Unit, Center for Cancer Research, Massachusetts General Hospital
    Center for Computational and Integrative Biology, Massachusetts General Hospital
    Harvard Medical School)

  • Moira M. Welch

    (Molecular Pathology Unit, Center for Cancer Research, Massachusetts General Hospital
    Center for Computational and Integrative Biology, Massachusetts General Hospital)

  • Alexander A. Sousa

    (Molecular Pathology Unit, Center for Cancer Research, Massachusetts General Hospital
    Center for Computational and Integrative Biology, Massachusetts General Hospital)

  • Lucas B. Harrington

    (University of California)

  • Samuel H. Sternberg

    (University of California
    Columbia University)

  • J. Keith Joung

    (Molecular Pathology Unit, Center for Cancer Research, Massachusetts General Hospital
    Center for Computational and Integrative Biology, Massachusetts General Hospital
    Harvard Medical School)

  • Ahmet Yildiz

    (University of California
    University of California)

  • Jennifer A. Doudna

    (University of California
    University of California
    Howard Hughes Medical Institute, University of California
    Lawrence Berkeley National Laboratory)

Abstract

A new engineered version of SpCas9, called HypaCas9, displays enhanced accuracy of editing without significant loss of efficiency at the desired target.

Suggested Citation

  • Janice S. Chen & Yavuz S. Dagdas & Benjamin P. Kleinstiver & Moira M. Welch & Alexander A. Sousa & Lucas B. Harrington & Samuel H. Sternberg & J. Keith Joung & Ahmet Yildiz & Jennifer A. Doudna, 2017. "Enhanced proofreading governs CRISPR–Cas9 targeting accuracy," Nature, Nature, vol. 550(7676), pages 407-410, October.
    • Handle: RePEc:nat:nature:v:550:y:2017:i:7676:d:10.1038_nature24268
    DOI: 10.1038/nature24268
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