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The CRISPR-associated DNA-cleaving enzyme Cpf1 also processes precursor CRISPR RNA

Author

Listed:
  • Ines Fonfara

    (The Laboratory for Molecular Infection Medicine Sweden (MIMS), Umeå Centre for Microbial Research (UCMR), Umeå University
    Helmholtz Centre for Infection Research
    Max Planck Institute for Infection Biology)

  • Hagen Richter

    (Helmholtz Centre for Infection Research
    Max Planck Institute for Infection Biology)

  • Majda Bratovič

    (Helmholtz Centre for Infection Research
    Max Planck Institute for Infection Biology
    Hannover Medical School)

  • Anaïs Le Rhun

    (The Laboratory for Molecular Infection Medicine Sweden (MIMS), Umeå Centre for Microbial Research (UCMR), Umeå University
    Helmholtz Centre for Infection Research
    Max Planck Institute for Infection Biology)

  • Emmanuelle Charpentier

    (The Laboratory for Molecular Infection Medicine Sweden (MIMS), Umeå Centre for Microbial Research (UCMR), Umeå University
    Helmholtz Centre for Infection Research
    Max Planck Institute for Infection Biology
    Hannover Medical School)

Abstract

The CRISPR-associated protein Cpf1 from Francisella novicida is a novel enzyme with specific, dual-endoribonuclease–endonuclease activities in precursor crRNA processing and crRNA-programmable cleavage of target DNA.

Suggested Citation

  • Ines Fonfara & Hagen Richter & Majda Bratovič & Anaïs Le Rhun & Emmanuelle Charpentier, 2016. "The CRISPR-associated DNA-cleaving enzyme Cpf1 also processes precursor CRISPR RNA," Nature, Nature, vol. 532(7600), pages 517-521, April.
    • Handle: RePEc:nat:nature:v:532:y:2016:i:7600:d:10.1038_nature17945
    DOI: 10.1038/nature17945
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    Cited by:

    1. Shunsuke Kawasaki & Hiroki Ono & Moe Hirosawa & Takeru Kuwabara & Shunsuke Sumi & Suji Lee & Knut Woltjen & Hirohide Saito, 2023. "Programmable mammalian translational modulators by CRISPR-associated proteins," Nature Communications, Nature, vol. 14(1), pages 1-17, December.
    2. Margot Karlikow & Evan Amalfitano & Xiaolong Yang & Jennifer Doucet & Abigail Chapman & Peivand Sadat Mousavi & Paige Homme & Polina Sutyrina & Winston Chan & Sofia Lemak & Alexander F. Yakunin & Adam, 2023. "CRISPR-induced DNA reorganization for multiplexed nucleic acid detection," Nature Communications, Nature, vol. 14(1), pages 1-11, December.
    3. Santosh R. Rananaware & Emma K. Vesco & Grace M. Shoemaker & Swapnil S. Anekar & Luke Samuel W. Sandoval & Katelyn S. Meister & Nicolas C. Macaluso & Long T. Nguyen & Piyush K. Jain, 2023. "Programmable RNA detection with CRISPR-Cas12a," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
    4. Pei Liu & Josquin Foiret & Yinglin Situ & Nisi Zhang & Aris J. Kare & Bo Wu & Marina N. Raie & Katherine W. Ferrara & Lei S. Qi, 2023. "Sonogenetic control of multiplexed genome regulation and base editing," Nature Communications, Nature, vol. 14(1), pages 1-11, December.
    5. Qichen Yuan & Xue Gao, 2022. "Multiplex base- and prime-editing with drive-and-process CRISPR arrays," Nature Communications, Nature, vol. 13(1), pages 1-13, December.

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