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c-kit+ cells minimally contribute cardiomyocytes to the heart

Author

Listed:
  • Jop H. van Berlo

    (Cincinnati Children’s Hospital Medical Center
    Lillehei Heart Institute, University of Minnesota)

  • Onur Kanisicak

    (Cincinnati Children’s Hospital Medical Center)

  • Marjorie Maillet

    (Cincinnati Children’s Hospital Medical Center)

  • Ronald J. Vagnozzi

    (Cincinnati Children’s Hospital Medical Center)

  • Jason Karch

    (Cincinnati Children’s Hospital Medical Center)

  • Suh-Chin J. Lin

    (Cincinnati Children’s Hospital Medical Center)

  • Ryan C. Middleton

    (Cedars-Sinai Heart Institute, 8700 Beverly Boulevard)

  • Eduardo Marbán

    (Cedars-Sinai Heart Institute, 8700 Beverly Boulevard)

  • Jeffery D. Molkentin

    (Cincinnati Children’s Hospital Medical Center
    Howard Hughes Medical Institute, Cincinnati Children’s Hospital Medical Center)

Abstract

If and how the heart regenerates after an injury event is highly debated. c-kit-expressing cardiac progenitor cells have been reported as the primary source for generation of new myocardium after injury. Here we generated two genetic approaches in mice to examine whether endogenous c-kit+ cells contribute differentiated cardiomyocytes to the heart during development, with ageing or after injury in adulthood. A complementary DNA encoding either Cre recombinase or a tamoxifen-inducible MerCreMer chimaeric protein was targeted to the Kit locus in mice and then bred with reporter lines to permanently mark cell lineage. Endogenous c-kit+ cells did produce new cardiomyocytes within the heart, although at a percentage of approximately 0.03 or less, and if a preponderance towards cellular fusion is considered, the percentage falls to below approximately 0.008. By contrast, c-kit+ cells amply generated cardiac endothelial cells. Thus, endogenous c-kit+ cells can generate cardiomyocytes within the heart, although probably at a functionally insignificant level.

Suggested Citation

  • Jop H. van Berlo & Onur Kanisicak & Marjorie Maillet & Ronald J. Vagnozzi & Jason Karch & Suh-Chin J. Lin & Ryan C. Middleton & Eduardo Marbán & Jeffery D. Molkentin, 2014. "c-kit+ cells minimally contribute cardiomyocytes to the heart," Nature, Nature, vol. 509(7500), pages 337-341, May.
    • Handle: RePEc:nat:nature:v:509:y:2014:i:7500:d:10.1038_nature13309
    DOI: 10.1038/nature13309
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    Cited by:

    1. Georges Makhoul & Kashif Khan & Renzo Cecere & Bin Yu & Adel Schwertani, 2019. "Triggered Cardiogenesis in Wingless 5a Treated Amniotic Mesenchymal Stromal Cells," Biomedical Journal of Scientific & Technical Research, Biomedical Research Network+, LLC, vol. 16(4), pages 12205-12213, April.
    2. Chennuru Veeranjaneyulu & Gangapatnam Subrahmanayam, 2018. "The Potential Role of Human Induced Pluripotent Stem Cells (Hipsc) - Derived In Cardiomyocytes in Cardiovascular Disease – Review," Current Trends in Biomedical Engineering & Biosciences, Juniper Publishers Inc., vol. 11(3), pages 67-69, January.

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