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DNA end resection by Dna2–Sgs1–RPA and its stimulation by Top3–Rmi1 and Mre11–Rad50–Xrs2

Author

Listed:
  • Petr Cejka

    (University of California, Davis, Davis, California 95616-8665, USA
    University of California, Davis, Davis, California 95616-8665, USA)

  • Elda Cannavo

    (University of California, Davis, Davis, California 95616-8665, USA
    University of California, Davis, Davis, California 95616-8665, USA)

  • Piotr Polaczek

    (California Institute of Technology)

  • Taro Masuda-Sasa

    (California Institute of Technology)

  • Subhash Pokharel

    (California Institute of Technology)

  • Judith L. Campbell

    (California Institute of Technology)

  • Stephen C. Kowalczykowski

    (University of California, Davis, Davis, California 95616-8665, USA
    University of California, Davis, Davis, California 95616-8665, USA)

Abstract

Priming DNA for repair When DNA damage introduces double-strand breaks, the ends formed must undergo processing to prepare them for repair. In related studies by the Sung and Kowalczykowski laboratories, this processing reaction has been replicated in vitro using yeast proteins. Processing minimally requires the activities of a helicase, a nuclease and a single-strand binding protein, although the reaction is enhanced by further addition of three factors that help target the core complex and enhance the unwinding activity.

Suggested Citation

  • Petr Cejka & Elda Cannavo & Piotr Polaczek & Taro Masuda-Sasa & Subhash Pokharel & Judith L. Campbell & Stephen C. Kowalczykowski, 2010. "DNA end resection by Dna2–Sgs1–RPA and its stimulation by Top3–Rmi1 and Mre11–Rad50–Xrs2," Nature, Nature, vol. 467(7311), pages 112-116, September.
    • Handle: RePEc:nat:nature:v:467:y:2010:i:7311:d:10.1038_nature09355
    DOI: 10.1038/nature09355
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