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Singh et al. reply

Author

Listed:
  • Sanjay K. Singh

    (The University of Texas M. D. Anderson Cancer Center)

  • Mohamedi N. Kagalwala

    (The University of Texas M. D. Anderson Cancer Center
    Present address: Laboratory of Genetics, Salk Institute for Biological Studies, La Jolla, California 92037, USAsmajumder@mdanderson.org)

  • Jan Parker-Thornburg

    (The University of Texas M. D. Anderson Cancer Center)

  • Henry Adams

    (The University of Texas M. D. Anderson Cancer Center)

  • Sadhan Majumder

    (The University of Texas M. D. Anderson Cancer Center)

Abstract

Replying to: H. F. Jørgensen & A. G. Fisher Nature 467, doi:10.1038/nature09305 (2010) Jørgensen and Fisher 1 suggest that the discrepancy with our results2 could be attributable to our use of E14Tg2a (or its derivatives, such as OS25) rather than E14Tg2a.4 as the parental control line for the REST+/− cells (YHC and RRC). We have now reconfirmed our use of E14Tg2a.4 clonal cells as the control cells. Also, we have found that the YHC and RRC cells used in our experiments2, originally purchased from Bay Genomics, differ from the YHC and RRC cells used by Jørgensen and Fisher1 with respect to pluripotency-based on alkaline phosphatase/self-renewal assays (N. Song and S.K.S., unpublished results). We are currently using other assays to confirm these observations.

Suggested Citation

  • Sanjay K. Singh & Mohamedi N. Kagalwala & Jan Parker-Thornburg & Henry Adams & Sadhan Majumder, 2010. "Singh et al. reply," Nature, Nature, vol. 467(7311), pages 5-5, September.
    • Handle: RePEc:nat:nature:v:467:y:2010:i:7311:d:10.1038_nature09306
    DOI: 10.1038/nature09306
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