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Can controversies be put to REST?

Author

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  • Helle F. Jørgensen

    (Lymphocyte Development Group, MRC Clinical Sciences Centre, Imperial College School of Medicine, Hammersmith Hospital Campus
    MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK hjorgen@nimr.mrc.ac.uk)

  • Amanda G. Fisher

    (Lymphocyte Development Group, MRC Clinical Sciences Centre, Imperial College School of Medicine, Hammersmith Hospital Campus)

Abstract

Arising from: S. K. Singh et al. Nature 453, 223–227 (2008)10.1038/nature06863 ; Singh et al. reply The contribution of REST to embryonic stem (ES) cell pluripotency has been uncertain. Two years ago, Singh et al.1 claimed that Rest+/− and REST knock-down ES cells expressed reduced levels of pluripotency markers1, in contrast to a prior2 and subsequent reports3,4,5,6. To understand the basis of this difference, we analysed the YHC334 (YHC) and RRC160 (RRC) gene-trap ES cell lines used by Singh et al.1, obtained directly from BayGenomics. Both REST mutant lines generated REST–βGeo fusion proteins, but expressed pluripotency genes at levels similar to appropriately matched parental wild ES cells, consistent with expression being REST–independent.

Suggested Citation

  • Helle F. Jørgensen & Amanda G. Fisher, 2010. "Can controversies be put to REST?," Nature, Nature, vol. 467(7311), pages 3-4, September.
    • Handle: RePEc:nat:nature:v:467:y:2010:i:7311:d:10.1038_nature09305
    DOI: 10.1038/nature09305
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