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Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes

Author

Listed:
  • Beate Neumann

    (MitoCheck Project Group,)

  • Thomas Walter

    (MitoCheck Project Group,)

  • Jean-Karim Hériché

    (Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1HH, UK
    Present addresses: MitoCheck Project Group, European Molecular Biology Laboratory (EMBL), Meyerhofstrasse 1, D-69117 Heidelberg, Germany (J.-K.H.); BIOQUANT Centre University Heidelberg, INF 267, D-69120 Heidelberg, Germany (H.E.); 3-V Biosciences GmbH, Wagistrasse 27, 8952 Schlieren, Switzerland (P.R.); Institute of Biochemistry, Swiss Federal Institute of Technology Zurich (ETHZ), Schafmattstrasse 18, CH-8093 Zurich, Switzerland (M.H.); Karlsruhe Institute of Technology KIT, Herrmann-von-Helmholtz Platz 1, D-76344 Eggenstein-Leopoldshafen, Germany (U.L.).)

  • Jutta Bulkescher

    (MitoCheck Project Group,)

  • Holger Erfle

    (MitoCheck Project Group,
    Cell Biology/Biophysics Units, Structural and,
    Present addresses: MitoCheck Project Group, European Molecular Biology Laboratory (EMBL), Meyerhofstrasse 1, D-69117 Heidelberg, Germany (J.-K.H.); BIOQUANT Centre University Heidelberg, INF 267, D-69120 Heidelberg, Germany (H.E.); 3-V Biosciences GmbH, Wagistrasse 27, 8952 Schlieren, Switzerland (P.R.); Institute of Biochemistry, Swiss Federal Institute of Technology Zurich (ETHZ), Schafmattstrasse 18, CH-8093 Zurich, Switzerland (M.H.); Karlsruhe Institute of Technology KIT, Herrmann-von-Helmholtz Platz 1, D-76344 Eggenstein-Leopoldshafen, Germany (U.L.).)

  • Christian Conrad

    (MitoCheck Project Group,
    Cell Biology/Biophysics Units, Structural and,)

  • Phill Rogers

    (MitoCheck Project Group,
    Present addresses: MitoCheck Project Group, European Molecular Biology Laboratory (EMBL), Meyerhofstrasse 1, D-69117 Heidelberg, Germany (J.-K.H.); BIOQUANT Centre University Heidelberg, INF 267, D-69120 Heidelberg, Germany (H.E.); 3-V Biosciences GmbH, Wagistrasse 27, 8952 Schlieren, Switzerland (P.R.); Institute of Biochemistry, Swiss Federal Institute of Technology Zurich (ETHZ), Schafmattstrasse 18, CH-8093 Zurich, Switzerland (M.H.); Karlsruhe Institute of Technology KIT, Herrmann-von-Helmholtz Platz 1, D-76344 Eggenstein-Leopoldshafen, Germany (U.L.).)

  • Ina Poser

    (Max Planck Institute for Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, D-01307 Dresden, Germany)

  • Michael Held

    (MitoCheck Project Group,
    Present addresses: MitoCheck Project Group, European Molecular Biology Laboratory (EMBL), Meyerhofstrasse 1, D-69117 Heidelberg, Germany (J.-K.H.); BIOQUANT Centre University Heidelberg, INF 267, D-69120 Heidelberg, Germany (H.E.); 3-V Biosciences GmbH, Wagistrasse 27, 8952 Schlieren, Switzerland (P.R.); Institute of Biochemistry, Swiss Federal Institute of Technology Zurich (ETHZ), Schafmattstrasse 18, CH-8093 Zurich, Switzerland (M.H.); Karlsruhe Institute of Technology KIT, Herrmann-von-Helmholtz Platz 1, D-76344 Eggenstein-Leopoldshafen, Germany (U.L.).)

  • Urban Liebel

    (MitoCheck Project Group,
    Present addresses: MitoCheck Project Group, European Molecular Biology Laboratory (EMBL), Meyerhofstrasse 1, D-69117 Heidelberg, Germany (J.-K.H.); BIOQUANT Centre University Heidelberg, INF 267, D-69120 Heidelberg, Germany (H.E.); 3-V Biosciences GmbH, Wagistrasse 27, 8952 Schlieren, Switzerland (P.R.); Institute of Biochemistry, Swiss Federal Institute of Technology Zurich (ETHZ), Schafmattstrasse 18, CH-8093 Zurich, Switzerland (M.H.); Karlsruhe Institute of Technology KIT, Herrmann-von-Helmholtz Platz 1, D-76344 Eggenstein-Leopoldshafen, Germany (U.L.).)

  • Cihan Cetin

    (Cell Biology/Biophysics Units, Structural and,)

  • Frank Sieckmann

    (Leica Microsystems CMS GmbH, Am Friedensplatz 3, D-68165 Mannheim, Germany)

  • Gregoire Pau

    (European Bioinformatics Institute, European Molecular Biology Laboratory)

  • Rolf Kabbe

    (German Cancer Research Center, Im Neuenheimer Feld 267, D-69120 Heidelberg, Germany)

  • Annelie Wünsche

    (Gene Expression and,)

  • Venkata Satagopam

    (Computational Biology Unit, European Molecular Biology Laboratory (EMBL), Meyerhofstrasse 1, D-69117 Heidelberg, Germany)

  • Michael H. A. Schmitz

    (Institute of Biochemistry, Swiss Federal Institute of Technology Zurich (ETHZ), Schafmattstrasse 18, CH-8093 Zurich, Switzerland)

  • Catherine Chapuis

    (Cell Biology/Biophysics Units, Structural and,)

  • Daniel W. Gerlich

    (Institute of Biochemistry, Swiss Federal Institute of Technology Zurich (ETHZ), Schafmattstrasse 18, CH-8093 Zurich, Switzerland)

  • Reinhard Schneider

    (Computational Biology Unit, European Molecular Biology Laboratory (EMBL), Meyerhofstrasse 1, D-69117 Heidelberg, Germany)

  • Roland Eils

    (German Cancer Research Center, Im Neuenheimer Feld 267, D-69120 Heidelberg, Germany)

  • Wolfgang Huber

    (European Bioinformatics Institute, European Molecular Biology Laboratory)

  • Jan-Michael Peters

    (Institute for Molecular Pathology, Dr Bohr Gasse 7, A-1030 Vienna, Austria)

  • Anthony A. Hyman

    (Max Planck Institute for Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, D-01307 Dresden, Germany)

  • Richard Durbin

    (Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1HH, UK)

  • Rainer Pepperkok

    (Cell Biology/Biophysics Units, Structural and,)

  • Jan Ellenberg

    (Gene Expression and,)

Abstract

Despite our rapidly growing knowledge about the human genome, we do not know all of the genes required for some of the most basic functions of life. To start to fill this gap we developed a high-throughput phenotypic screening platform combining potent gene silencing by RNA interference, time-lapse microscopy and computational image processing. We carried out a genome-wide phenotypic profiling of each of the ∼21,000 human protein-coding genes by two-day live imaging of fluorescently labelled chromosomes. Phenotypes were scored quantitatively by computational image processing, which allowed us to identify hundreds of human genes involved in diverse biological functions including cell division, migration and survival. As part of the Mitocheck consortium, this study provides an in-depth analysis of cell division phenotypes and makes the entire high-content data set available as a resource to the community.

Suggested Citation

  • Beate Neumann & Thomas Walter & Jean-Karim Hériché & Jutta Bulkescher & Holger Erfle & Christian Conrad & Phill Rogers & Ina Poser & Michael Held & Urban Liebel & Cihan Cetin & Frank Sieckmann & Grego, 2010. "Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes," Nature, Nature, vol. 464(7289), pages 721-727, April.
    • Handle: RePEc:nat:nature:v:464:y:2010:i:7289:d:10.1038_nature08869
    DOI: 10.1038/nature08869
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